Western Blot (WB)
(Figure 1. Western blot analysis of PFKFB2 using anti-PFKFB2 antibody (MBS1753720).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human A431 whole cell lysatesLane 6: human PC-3 whole cell lysatesLane 7: rat heart tissue lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse heart tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PFKFB2 antigen affinity purified polyclonal antibody (Catalog # MBS1753720) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PFKFB2 at approximately 58KD. The expected band size for PFKFB2 is at 58KD. )
Rabbit PFKFB2 Polyclonal Antibody | anti-PFKFB2 antibody
Anti-PFKFB2 Antibody
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of PFKFB2 using anti-PFKFB2 antibody (MBS1753720).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human A431 whole cell lysatesLane 6: human PC-3 whole cell lysatesLane 7: rat heart tissue lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse heart tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PFKFB2 antigen affinity purified polyclonal antibody (Catalog # MBS1753720) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PFKFB2 at approximately 58KD. The expected band size for PFKFB2 is at 58KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of PFKFB2 using anti-PFKFB2 antibody (MBS1753720).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human A431 whole cell lysatesLane 6: human PC-3 whole cell lysatesLane 7: rat heart tissue lysatesLane 8: rat PC-12 whole cell lysatesLane 9: mouse heart tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-PFKFB2 antigen affinity purified polyclonal antibody (Catalog # MBS1753720) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for PFKFB2 at approximately 58KD. The expected band size for PFKFB2 is at 58KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of PFKFB2 using anti- PFKFB2 antibody (MBS1753720).PFKFB2 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PFKFB2 Antibody (MBS1753720) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of PFKFB2 using anti- PFKFB2 antibody (MBS1753720).PFKFB2 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PFKFB2 Antibody (MBS1753720) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of A431 cells using anti-PFKFB2 antibody (MBS1753720).Overlay histogram showing A431 cells stained with MBS1753720 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB2 Antibody (MBS1753720, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of A431 cells using anti-PFKFB2 antibody (MBS1753720).Overlay histogram showing A431 cells stained with MBS1753720 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB2 Antibody (MBS1753720, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of CACO-2 cells using anti-PFKFB2 antibody (MBS1753720).Overlay histogram showing CACO-2 cells stained with MBS1753720 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB2 Antibody (MBS1753720, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of CACO-2 cells using anti-PFKFB2 antibody (MBS1753720).Overlay histogram showing CACO-2 cells stained with MBS1753720 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB2 Antibody (MBS1753720, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Heine-Suner, D., Diaz-Guillen, M. A., Lange, A. J., Rodriguez de Cordoba, S. Sequence and structure of the human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase heart isoform gene (PFKFB2). Europ. J. Biochem. 254: 103-110, 1998.
3. Hilliker, C. E., Darville, M. I., Aly, M. S., Chikri, M., Szpirer, C., Marynen, P., Rousseau, G. G., Cassiman, J. -J. Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. Genomics 10: 867-873, 1991.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described.
Protein type: EC 3.1.3.46; Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.105; Kinase, other; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: cytosol
Molecular Function: protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; protein kinase binding; ATP binding
Biological Process: glucose catabolic process; glycolysis; dephosphorylation; lactate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; positive regulation of insulin secretion; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; positive regulation of glucokinase activity; fructose metabolic process
Research Articles on PFKFB2
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Product Notes
The PFKFB2 pfkfb2 (Catalog #AAA1753720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PFKFB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the PFKFB2 pfkfb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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