Western Blot (WB)
(Western Blot; Sample: Recombinant Pgp, Rat.)
Rabbit anti-Rat Permeability Glycoprotein (Pgp) Polyclonal Antibody | anti-Pgp antibody
Polyclonal Antibody to Permeability Glycoprotein (Pgp)
Gene Names
Abcb1b; Mdr1; Pgy1; Abcb1; mdr1b
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Permeability Glycoprotein (Pgp); Polyclonal Antibody; Polyclonal Antibody to Permeability Glycoprotein (Pgp); CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette; Sub-Family B(MDR/TAP); Member 1; Colchicin Sensitivity; anti-Pgp antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Pgp. It has been selected for its ability to recognize Pgp in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.89mg/ml (varies by lot)
Sequence
Ala999~Ser1277
Applicable Applications for anti-Pgp antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:450-1800
IHC: 5-20ug/mL; 1:45-180
ICC: 5-20ug/mL; 1:45-180
IHC: 5-20ug/mL; 1:45-180
ICC: 5-20ug/mL; 1:45-180
Immunogen Sequence
Ala999~Ser1277
Cross Reactivity
Rat
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant Pgp, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant Pgp, Rat.)
Western Blot (WB)
(Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Cerebellum lysatePrimary Ab: 1ug/ml Rabbit Anti-Rat Pgp AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Cerebellum lysatePrimary Ab: 1ug/ml Rabbit Anti-Rat Pgp AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10ug/ml Rabbit Anti-Rat Pgp Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10ug/ml Rabbit Anti-Rat Pgp Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Product Categories/Family for anti-Pgp antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
141,387 Da
NCBI Official Full Name
Multidrug resistance protein 1
NCBI Official Synonym Full Names
ATP-binding cassette, subfamily B (MDR/TAP), member 1B
NCBI Official Symbol
Abcb1b
NCBI Official Synonym Symbols
Mdr1; Pgy1; Abcb1; mdr1b
NCBI Protein Information
multidrug resistance protein 1
UniProt Protein Name
Multidrug resistance protein 1
Protein Family
UniProt Gene Name
Abcb1
UniProt Synonym Gene Names
Mdr1; Mdr1b; Pgy1
NCBI Description
member of the ATP-binding cassette (ABC) protein superfamily; may play a role in drug disposition [RGD, Feb 2006]
Uniprot Description
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Research Articles on Pgp
1. conclusion, MDR1 and BCRP are expressed on apical membranes of the rodent placental SynT-II layer.
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Product Notes
The Pgp abcb1 (Catalog #AAA2112143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Permeability Glycoprotein (Pgp) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Permeability Glycoprotein (Pgp) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:450-1800 IHC: 5-20ug/mL; 1:45-180 ICC: 5-20ug/mL; 1:45-180. Researchers should empirically determine the suitability of the Pgp abcb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala999~Ser 1277. It is sometimes possible for the material contained within the vial of "Permeability Glycoprotein (Pgp), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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