Rabbit PERK Polyclonal Antibody | anti-PERK antibody

PERK Rabbit pAb

Cat. Num. AAA4755312

• Gene Names: EIF2AK3; PEK; WRS; PERK
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry, ELISA
• Purity: Affinity purified
• Synonyms: PERK; Polyclonal Antibody; PERK Rabbit pAb; EIF2AK3; PEK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; anti-PERK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PERK Polyclonal Antibody detects endogenous levels of PERK protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-PERK antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), ELISA (EIA)
• Application Notes: IF: 1:50-200; WB: 1:500-1:2000; IHC: 1:100-1:300; ELISA: 1:40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996
• Sequence Similarity: Contains 1 protein kinase domain.
• Conjugation: Unconjugated
• Cell Position: Endoplasmic reticulum, Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of lysates from MCF-7 cells, using PERK Antibody. The lane on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells using PERK Polyclonal Antibody(green, 1:200 diluted))

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue using PERK Polyclonal Antibody diluted at 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Rat-liver tissue using PERK Polyclonal Antibody diluted at 1:200)

Product Categories/Family for anti-PERK antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 134304838
• NCBI GeneID: 9451
• NCBI Accession #: NP_004827.4
• NCBI GenBank Nucleotide #: NM_004836.5
• UniProt Accession #: Q9NZJ5; O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9
• Molecular Weight: 125,216 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 3
• NCBI Official Symbol: EIF2AK3
• NCBI Official Synonym Symbols: PEK; WRS; PERK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; pancreatic EIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; eukaryotic translation initiation factor 2 alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Gene Name: EIF2AK3
• UniProt Synonym Gene Names: PEK; PERK; HsPEK
• UniProt Entry Name: E2AK3_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: EC 2.7.11.1; Membrane protein, integral; Translation; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family; PEK subfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: fat cell differentiation; lactation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; ER overload response; protein homooligomerization; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus