Goat Pericentrin 1 Polyclonal Antibody | anti-PCNT antibody

Pericentrin 1 (PCNT1, PCN, PCNT, Pericentrin 1, Frount, Nucleoporin Nup75, Nucleoporin 85kD, NUP85)

Cat. Num. AAA615319

• Gene Names: PCNT; KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Pericentrin 1; Polyclonal Antibody; Pericentrin 1 (PCNT1; PCN; PCNT; Frount; Nucleoporin Nup75; Nucleoporin 85kD; NUP85); Anti -Pericentrin 1 (PCNT1; anti-PCNT antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KD band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). Species Crossreactivity: human, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PCNT antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75kD band observed in HeLa lysates (calculated MW of 75kD according to NP_079120). Recommended concentration: 1-3ug/ml., 1ug/ml staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher.
• Immunogen: C-ARAIIREGSLEGS, C Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-PCNT antibody

Antibodies; Abs to Mitosis Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 81295809
• NCBI GeneID: 5116
• NCBI Accession #: NP_006022.3
• NCBI GenBank Nucleotide #: NM_006031.5
• UniProt Accession #: O95613; O43152; Q7Z7C9
• Molecular Weight: 378,037 Da
• NCBI Official Full Name: pericentrin
• NCBI Official Synonym Full Names: pericentrin
• NCBI Official Symbol: PCNT
• NCBI Official Synonym Symbols: KEN; PCN; MOPD2; PCNT2; PCNTB; PCTN2; SCKL4
• NCBI Protein Information: pericentrin; kendrin; pericentrin B; pericentrin-2; pericentrin-B; pericentrin-380
• UniProt Protein Name: Pericentrin
• Protein Family: Pericentrin
• UniProt Gene Name: PCNT
• UniProt Synonym Gene Names: KIAA0402; PCNT2
• UniProt Entry Name: PCNT_HUMAN

NCBI Description

The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled-coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus. The protein interacts with the microtubule nucleation component gamma-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell-cycle progression. Mutations in this gene cause Seckel syndrome-4 and microcephalic osteodysplastic primordial dwarfism type II. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNT: Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2); also known as osteodysplastic primordial dwarfism type 2. Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: centriole; microtubule; centrosome; pericentriolar material; intercellular bridge; membrane; cytoplasm; cytosol

Molecular Function: calmodulin binding; protein binding

Biological Process: cerebellar cortex morphogenesis; in utero embryonic development; olfactory bulb development; organelle organization and biogenesis; multicellular organism growth; brain morphogenesis; neuron migration; cilium biogenesis; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; spindle organization and biogenesis; limb morphogenesis; negative regulation of apoptosis

Disease: Microcephalic Osteodysplastic Primordial Dwarfism, Type Ii