Western Blot (WB)
(Anti- PER2 Picoband antibody, MBS177843, Western blottingAll lanes: Anti PER2 (MBS177843) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 136KDObserved bind size: 136KD )
anti-Human PER2 Polyclonal Antibody | anti-PER2 antibody
Anti-PER2 Antibody
WB: The detection limit for PER2 is approximately 0.1ng/lane under reducing conditions.
Tested Species: In-House tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by the end users.
Antibody can be supported chemiluminescence kit MBS176460 in Western Blot.
Western Blot (WB)
(Anti- PER2 Picoband antibody, MBS177843, Western blottingAll lanes: Anti PER2 (MBS177843) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 136KDObserved bind size: 136KD )
Western Blot (WB)
(Anti- PER2 Picoband antibody, MBS177843, Western blottingAll lanes: Anti PER2 (MBS177843) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 136KDObserved bind size: 136KD )
Background: The PER2 gene encodes the period circadian protein homolog 2 protein in humans. This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. PER2 is mapped to 2q37.3. It has been showed that PER2 can positively stimulate activity of the Bmal1-Npas2 transcription complex and, in turn, Npas2 transcriptionally regulates delta-aminolevulinate synthase. PER2 is involved in a negative-feedback loop that attenuates estrogen stimulation and links the circadian cycle to estrogen receptor signaling. The rhythmic levels of PER2 are critical for circadian oscillations in cells and intact organisms. What's more, the PER2 gene also functions in tumor suppression by regulating DNA damage-responsive pathways.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]
Uniprot Description
PER2: a transcription factor belonging to the basic helix-loop-helix (bHLH) family. A member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hr rhythm in the suprachiasmatic nucleus and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between per1 and per2 oscillations. The expression rhythms appear to originate from retina.
Protein type: Nucleolus; Nuclear receptor co-regulator; Transcription factor
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm
Molecular Function: protein binding; transcription coactivator activity; ubiquitin binding
Biological Process: circadian regulation of gene expression; circadian rhythm; fatty acid metabolic process; gluconeogenesis; glycogen biosynthetic process; lactate biosynthetic process; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of cell cycle; regulation of circadian rhythm; regulation of glutamate uptake during transmission of nerve impulse; regulation of insulin secretion; regulation of neurogenesis; regulation of vasoconstriction; transcription, DNA-dependent; white fat cell differentiation
Disease: Advanced Sleep Phase Syndrome, Familial, 1
Research Articles on PER2
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Product Notes
The PER2 per2 (Catalog #AAA177843) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-PER2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PER2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Human WB: The detection limit for PER2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-House tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by the end users. Antibody can be supported chemiluminescence kit MBS176460 in Western Blot. Researchers should empirically determine the suitability of the PER2 per2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PER2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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