Rabbit anti-Human PER1 Polyclonal Antibody | anti-PER1 antibody

PER1, CT (PER1, KIAA0482, PER, RIGUI, Period circadian protein homolog 1, Circadian clock protein PERIOD 1, Circadian pacemaker protein Rigui) (PE)

Cat. Num. AAA6332715

• Gene Names: PER1; PER; hPER; RIGUI
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PER1; Polyclonal Antibody; CT (PER1; KIAA0482; PER; RIGUI; Period circadian protein homolog 1; Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui) (PE); Circadian pacemaker protein Rigui; anti-PER1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PER1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1155-1183 amino acids from the C-terminal region of human PER1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PER1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PER1 antibody detected the PER1 protein (arrow).)

Related Product Information for anti-PER1 antibody

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.

Product Categories/Family for anti-PER1 antibody

Antibodies; KIAA; orf

References

Xia, H.C., et al. Can J Neurol Sci 37(3):365-370(2010). Soria, V., et al. Neuropsychopharmacology 35(6):1279-1289(2010). Utge, S.J., et al. PLoS ONE 5 (2), E9259 (2010): Zhu, Y., et al. Cancer Res. 69(24):9315-9322(2009). Pendergast, J.S., et al. J. Neurosci. 29(46):14681-14686(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 194097340
• NCBI GeneID: 5187
• NCBI Accession #: NP_002607.2
• NCBI GenBank Nucleotide #: NM_002616
• UniProt Accession #: O15534; B2RPA8; B4DI49; D3DTR3
• Molecular Weight: – Da
• NCBI Official Full Name: Homo sapiens period circadian clock 1 (PER1), mRNA
• NCBI Official Synonym Full Names: period circadian clock 1
• NCBI Official Symbol: PER1
• NCBI Official Synonym Symbols: PER; hPER; RIGUI
• NCBI Protein Information: period circadian protein homolog 1; Period, drosophila, homolog of; circadian clock protein PERIOD 1; circadian pacemaker protein RIGUI; hPER1; period homolog 1
• UniProt Protein Name: Period circadian protein homolog 1
• Protein Family: Protein
• UniProt Gene Name: PER1
• UniProt Synonym Gene Names: KIAA0482; PER; RIGUI; hPER1
• UniProt Entry Name: PER1_HUMAN

NCBI Description

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq, Jan 2014]

Uniprot Description

PER1: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation. Can bind heme. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; nucleus

Molecular Function: signal transducer activity; chromatin DNA binding; ubiquitin protein ligase binding; kinase binding; transcription factor binding

Biological Process: circadian rhythm; negative regulation of JNK cascade; response to cAMP; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; entrainment of circadian clock; regulation of sodium ion transport; circadian regulation of gene expression; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; signal transduction; regulation of hair cycle; entrainment of circadian clock by photoperiod