Rabbit PER-3 Polyclonal Antibody | anti-PER-3 antibody

PER-3 Polyclonal Antibody

Cat. Num. AAA3015429

• Gene Names: PER3; GIG13
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: PER-3; Polyclonal Antibody; PER-3 Polyclonal Antibody; 2810049O06Rik; Cell growth inhibiting gene 13 protein; Cell growth-inhibiting gene 13 protein; Circadian clock protein PERIOD 3; GIG13; gPER3; HGNC:8847; hPER3; mPer3; Per3; PER3_HUMAN; Period circadian protein homolog 3; Period homolog 3 (Drosophila); Period homolog 3; Drosophila; Period3.; anti-PER-3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: PER-3 Polyclonal Antibody detects endogenous levels of PER-3 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 148% Glycerol.

• Applicable Applications for anti-PER-3 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human PER-3:101-200/1201
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -67 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER-3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER-3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-PER-3 antibody

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 317373535
• NCBI GeneID: 8863
• UniProt Accession #: P56645; Q5H8X4; Q5H8X5; Q969K6; Q96S77; Q96S78; Q9C0J3; Q9NSP9; Q9UGU8
• Molecular Weight: 1201
• NCBI Official Full Name: Period circadian protein homolog 3
• NCBI Official Synonym Full Names: period circadian clock 3
• NCBI Official Symbol: PER3
• NCBI Official Synonym Symbols: GIG13
• NCBI Protein Information: period circadian protein homolog 3; hPER3; period 3; period homolog 3; period circadian protein 3; growth-inhibiting protein 13; circadian clock protein PERIOD 3; cell growth-inhibiting gene 13 protein
• UniProt Protein Name: Period circadian protein homolog 3
• UniProt Gene Name: PER3
• UniProt Synonym Gene Names: hPER3
• UniProt Entry Name: PER3_HUMAN

NCBI Description

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

PER3: Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: cytoplasm; nucleus

Molecular Function: signal transducer activity; protein binding; ubiquitin protein ligase binding; kinase binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; signal transduction; regulation of circadian sleep/wake cycle, sleep