Goat anti-Human PEPCK1 Polyclonal Antibody | anti-PCK1 antibody

PEPCK1, ID (Phosphoenolpyruvate Carboxykinase 1, PEPCK-1, Pck1, Phosphoenolpyruvate Carboxykinase Cytosolic [GTP], PEP Carboxykinase, PEPCK, PEPCKC, PEPCK-C, Phosphoenolpyruvate Carboxylase, HGNC:8724, MGC22652)

Cat. Num. AAA646836

• Gene Names: PCK1; PEPCK1; PEPCKC; PEPCK-C
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PEPCK1; Polyclonal Antibody; ID (Phosphoenolpyruvate Carboxykinase 1; PEPCK-1; Pck1; Phosphoenolpyruvate Carboxykinase Cytosolic [GTP]; PEP Carboxykinase; PEPCK; PEPCKC; PEPCK-C; Phosphoenolpyruvate Carboxylase; HGNC:8724; MGC22652); Anti -PEPCK1; anti-PCK1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PCK1. Species sequence homology: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PCK1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:8000; Immunohistochemistry (Formalin fixed paraffin embedded): 2ug/ml
• Immunogen: Synthetic peptide corresponding to C-HVNWFRKDKEGK from an internal region of human PCK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with PCK1 antibody at 2ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Liver (formalin-fixed, paraffin-embedded) stained with PCK1 antibody at 2ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PCK1 antibody

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq]

Product Categories/Family for anti-PCK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 187281517
• NCBI GeneID: 5105
• NCBI Accession #: NP_002582.3
• NCBI GenBank Nucleotide #: NM_002591.3
• UniProt Accession #: P35558; Q8TCA3; Q9UJD2; A8K437
• Molecular Weight: 69,195 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase, cytosolic
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 1 (soluble)
• NCBI Official Symbol: PCK1
• NCBI Official Synonym Symbols: PEPCK1; PEPCKC; PEPCK-C
• NCBI Protein Information: phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Protein Family: Protein kinase
• UniProt Gene Name: PCK1
• UniProt Synonym Gene Names: PEPCK1; PEPCK-C
• UniProt Entry Name: PCKGC_HUMAN

NCBI Description

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Catalytic activity: GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactor: Binds 1 manganese ion per subunit. Ref.12

Enzyme regulation: Enzyme activity is enhanced by acetylation. Ref.10

Pathway: Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure: Monomer.

Subcellular location: Cytoplasm.

Tissue specificity: Major sites of expression are liver, kidney and adipocytes.

Induction: Regulated by cAMP and insulin. Ref.9 Ref.10

Post-translational modification: Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2.Ubiquitination by UBR5 leads to proteasomal degradation.

Involvement in disease: Cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD) [MIM:261680]: Metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autopsy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Sequence similarities: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.