Rabbit anti-Human PENK Polyclonal Antibody | anti-PENK antibody

PENK, ID (PENK, Proenkephalin-A, Synenkephalin, Met-enkephalin, Opioid growth factor, PENK(114-133), PENK(143-183), Met-enkephalin-Arg-Gly-Leu, Leu-enkephalin, PENK(237-258), Met-enkephalin-Arg-Phe) (FITC)

Cat. Num. AAA6332697

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PENK; Polyclonal Antibody; ID (PENK; Proenkephalin-A; Synenkephalin; Met-enkephalin; Opioid growth factor; PENK(114-133); PENK(143-183); Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; PENK(237-258); Met-enkephalin-Arg-Phe) (FITC); Met-enkephalin-Arg-Phe; anti-PENK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PENK antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PENK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-182 amino acids from the Central region of human PENK.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PENK Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PENK antibody detected the PENK protein (arrow).)

Immunohistochemistry (IHC)

(PENK antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PENK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PENK Antibody (Center) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PENK antibody

Antibodies; Opioid Receptors; Agonists

NCBI and Uniprot Product Information

• NCBI GI #: 208879446
• NCBI GeneID: 5179
• NCBI Accession #: NP_001129162.1; NP_006202.1
• NCBI GenBank Nucleotide #: NM_001135690.1
• UniProt Accession #: P01210; Q6FHC6; Q6FHE6; B2RC23
• Molecular Weight: 30,787 Da
• NCBI Official Full Name: proenkephalin-A preproprotein
• NCBI Official Synonym Full Names: proenkephalin
• NCBI Official Symbol: PENK
• NCBI Protein Information: proenkephalin-A; enkephalin A; preproenkephalin
• UniProt Protein Name: Proenkephalin-A
• Protein Family: Proenkephalin
• UniProt Gene Name: PENK
• UniProt Synonym Gene Names: OGF
• UniProt Entry Name: PENK_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Uniprot Description

PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 8q23-q24

Cellular Component: extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; opioid peptide activity

Biological Process: startle response; behavioral fear response; neuropeptide signaling pathway; locomotory behavior; sensory perception of pain; signal transduction