Rabbit PEN2 Polyclonal Antibody | anti-PSENEN antibody

PEN2 Antibody

Cat. Num. AAA151154

• Gene Names: PSENEN; PEN2; PEN-2; MDS033; MSTP064
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PEN2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PEN2; Polyclonal Antibody; PEN2 Antibody; PEN-2; MDS033; MSTP064; Gamma-secretase subunit PEN-2; Presenilin enhancer protein 2; presenilin enhancer 2 homolog (C. elegans); anti-PSENEN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PEN2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 101

• Applicable Applications for anti-PSENEN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PEN2 antibody can be used for detection of PEN2 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PEN2 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human PEN2.
• Buffer: PEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PEN2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PEN2 in A-20 cell lysate with PEN2 antibody at (A) 0.5 and (B) 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of PEN2 in rat lung tissue with PEN2 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PEN2 in Rat Lung cells with PEN2 antibody at 20 μg/mL.)

Related Product Information for anti-PSENEN antibody

PEN2 Antibody: PEN2, in addition to presenilin, nicastrin, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease.

NCBI and Uniprot Product Information

• NCBI GI #: 28144920
• NCBI GeneID: 55851
• NCBI Accession #: NP_758844
• NCBI GenBank Nucleotide #: NM_172341.2
• UniProt Accession #: Q9NZ42; B2R5L9
• Molecular Weight: 12,029 Da
• NCBI Official Full Name: gamma-secretase subunit PEN-2
• NCBI Official Synonym Full Names: presenilin enhancer gamma secretase subunit
• NCBI Official Symbol: PSENEN
• NCBI Official Synonym Symbols: PEN2; PEN-2; MDS033; MSTP064
• NCBI Protein Information: gamma-secretase subunit PEN-2; presenilin enhancer 2 homolog; hematopoietic stem/progenitor cells protein MDS033
• UniProt Protein Name: Gamma-secretase subunit PEN-2
• Protein Family: Probable gamma-secretase
• UniProt Gene Name: PSENEN
• UniProt Synonym Gene Names: PEN2
• UniProt Entry Name: PEN2_HUMAN

NCBI Description

Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PSENEN: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PSENEN/PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Belongs to the PEN-2 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of catalytic activity; axon guidance; amyloid precursor protein catabolic process; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; ephrin receptor signaling pathway; Notch receptor processing; protein processing

Disease: Acne Inversa, Familial, 2