Rabbit PEF1 Polyclonal Antibody | anti-PEF1 antibody

PEF1 Antibody

Cat. Num. AAA9631379

• Gene Names: PEF1; ABP32; PEF1A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PEF1; Polyclonal Antibody; PEF1 Antibody; ABP32; PEF; PEF protein with a long N terminal hydrophobic domain; PEF protein with a long N-terminal hydrophobic domain; pef1; PEF1_HUMAN; PEF1A; Peflin; Penta EF hand domain containing protein 1; Penta-EF hand domain-containing protein 1; anti-PEF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PEF1 Antibody detects endogenous levels of PEF1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PEF1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PEF1.
• Conjugation: Unconjugated
• Post Translational Modifications: Ubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex.
• Subunit Structure: Heterodimer; heterodimerizes (via the EF-hand 5) with PDCD6. Dissociates from PDCD6 in presence of calcium.
• Function: Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR(KLHL12) complex, a complex involved in endoplasmic reticulum (ER)-Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidence shows that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31 (By similarity).
• Note: Membrane-associated in the presence of Ca(2+) (PubMed:11278427). Localizes to endoplasmic reticulum exit site (ERES) (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PEF1 antibody

Cytoplasm; Endoplasmic reticulum; Membrane; Peripheral membrane protein; Cytoplasmic vesicle; COPII-coated vesicle membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 6912582
• NCBI GeneID: 553115
• NCBI Accession #: NP_036524.1
• NCBI GenBank Nucleotide #: NM_012392.3
• UniProt Accession #: Q9UBV8
• Molecular Weight: 30,381 Da
• NCBI Official Full Name: peflin
• NCBI Official Synonym Full Names: penta-EF-hand domain containing 1
• NCBI Official Symbol: PEF1
• NCBI Official Synonym Symbols: ABP32; PEF1A
• NCBI Protein Information: peflin; PEF protein with a long N-terminal hydrophobic domain; penta-EF hand domain containing 1
• UniProt Protein Name: Peflin
• Protein Family: Peflin
• UniProt Gene Name: PEF1
• UniProt Synonym Gene Names: ABP32
• UniProt Entry Name: PEF1_HUMAN

NCBI Description

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]

Uniprot Description

PEF1: Heterodimer; heterodimerizes with PDCD6/ALG2. Dissociates from PDCD6/ALG2 in presence of Ca(2+).

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: membrane; cytoplasm

Molecular Function: protein dimerization activity; protein binding; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: proteolysis; response to calcium ion