Rabbit anti-Human PEDF Polyclonal Antibody | anti-SerpinF1 antibody

Anti-PEDF Antibody, Rabbit Polyclonal

Cat. Num. AAA8104891

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PEDF; Polyclonal Antibody; Anti-PEDF Antibody; Rabbit Polyclonal; SerpinF1/PEDF Antibody; Rabbit PAb; Antigen Affinity Purified; serpin peptidase inhibitor; clade F; member 1; Anti-EPC-1 Antibody; Anti-OI12 Antibody; Anti-OI6 Antibody; Anti-PIG35 Antibody; anti-SerpinF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SerpinF1/PEDF
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SerpinF1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate; ; ; ELISA: 1:5000-1:10,000; this antibody can be used at 1:5000-1:10000 with the appropriate secondary to detect Human SERPINF1.; ; ; Optimal concentrations/dilutions should be determined by the end user.
• Immunogen: Recombinant Human SerpinF1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human SerpinF1 (rh SerpinF1; NP_00260636; Met 1-Pro 418). SerpinF1 specific IgG was purified by human SerpinF1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%)It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SerpinF1 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate0.5 uL anti-SerpinF1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SerpinF1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size: 46 kDa)

Western Blot (WB)

(Anti-SerpinF1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.))

References

Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8. Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75. Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11. Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91. Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.5
• UniProt Accession #: P36955; Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• UniProt Protein Name: Pigment epithelium-derived factor
• Protein Family: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF
• UniProt Entry Name: PEDF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; negative regulation of inflammatory response; multicellular organismal development; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi