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Western Blot (WB) (Western blot analysis of PECI expression in Jurkat (A), Raji (B), A431 (C) whole cell lysates.)

Rabbit anti-Human PECI Polyclonal Antibody | anti-ECI2 antibody

Anti-PECI Antibody

Gene Names
ECI2; DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PECI; Polyclonal Antibody; Anti-PECI Antibody; DRS1; HCA88; Enoyl-CoA delta isomerase 2; mitochondrial; DRS-1; Delta(3); delta(2)-enoyl-CoA isomerase; D3; D2-enoyl-CoA isomerase; Diazepam-binding inhibitor-related protein 1; DBI-related protein 1; Dodecenoyl-CoA isomerase; Hepatocellular carcinoma-associated antigen 88; Peroxisomal 3; 2-trans-enoyl-CoA isomerase; pECI; Renal carcinoma antigen NY-REN-1; anti-ECI2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PECI protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
364
Applicable Applications for anti-ECI2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PECI. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of PECI expression in Jurkat (A), Raji (B), A431 (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of PECI expression in Jurkat (A), Raji (B), A431 (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PECI staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of PECI staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-ECI2 antibody
Rabbit polyclonal antibody to PECI

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,609 Da
NCBI Official Full Name
enoyl-CoA delta isomerase 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
enoyl-CoA delta isomerase 2
NCBI Official Symbol
ECI2
NCBI Official Synonym Symbols
DRS1; PECI; ACBD2; DRS-1; HCA88; dJ1013A10.3
NCBI Protein Information
enoyl-CoA delta isomerase 2, mitochondrial; DBI-related protein 1; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; renal carcinoma antigen NY-REN-1; delta(3),delta(2)-enoyl-CoA isomerase; peroxisomal D3,D2-enoyl-CoA isomerase; peroxisomal 3,2-trans-enoyl-CoA isomerase; acyl-Coenzyme A binding domain containing 2; diazepam-binding inhibitor-related protein 1; hepatocellular carcinoma-associated antigen 88
UniProt Protein Name
Enoyl-CoA delta isomerase 2, mitochondrial
Protein Family
UniProt Gene Name
ECI2
UniProt Synonym Gene Names
DRS1; HCA88; PECI; D3,D2-enoyl-CoA isomerase; DBI-related protein 1; pECI
UniProt Entry Name
ECI2_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]

Uniprot Description

PECI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; Lipid Metabolism - fatty acid; EC 5.3.3.8

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: nucleoplasm; peroxisomal matrix; intracellular membrane-bound organelle; membrane; mitochondrion

Molecular Function: acyl-CoA binding; dodecenoyl-CoA delta-isomerase activity; receptor binding

Biological Process: metabolic process; fatty acid catabolic process

Research Articles on ECI2

Similar Products

Product Notes

The ECI2 eci2 (Catalog #AAA820409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PECI Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PECI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the ECI2 eci2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PECI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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