Rabbit anti-Human PDX1 Polyclonal Antibody | anti-PDX1 antibody

PDX1, CT (Pyruvate Dehydrogenase Protein X Component, Mitochondrial, Dihydrolipoamide Dehydrogenase-binding Protein of Pyruvate Dehydrogenase Complex, Lipoyl-containing Pyruvate Dehydrogenase Complex Component X, E3-binding Protein, E3BP, PDHX) (Azide fre

Cat. Num. AAA6496181

• Gene Names: PDHX; E3BP; OPDX; PDX1; proX; DLDBP; PDHXD
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PDX1; Polyclonal Antibody; CT (Pyruvate Dehydrogenase Protein X Component; Mitochondrial; Dihydrolipoamide Dehydrogenase-binding Protein of Pyruvate Dehydrogenase Complex; Lipoyl-containing Pyruvate Dehydrogenase Complex Component X; E3-binding Protein; E3BP; PDHX) (Azide fre; anti-PDX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDX1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDX1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from selected from the C-terminal region of human PDX1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PDX1 Pab is used in Western blot to detect PDX1 in mouse heart tissue lysate.)

Western Blot (WB)

(Western blot analysis of PDX1 (arrow) using rabbit polyclonal PDX1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(PDX1 Antibody (R444) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDX1 antibody detected the PDX1 protein (arrow).)

Product Categories/Family for anti-PDX1 antibody

Antibodies; Protein Kinase Substrates

References

1. Ling, M., et al., Hum. Mol. Genet. 7(3):501-505 (1998). 2. Harris, R.A., et al., J. Biol. Chem. 272(32):19746-19751 (1997). 3. Murray, J., et al., FEBS Lett. 529(2-3), 173-178 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 203098753
• NCBI GeneID: 8050
• NCBI Accession #: NP_003468
• NCBI GenBank Nucleotide #: NM_003477.2
• UniProt Accession #: O00330; O60221; Q96FV8; Q99783; B4DW62; D3DR11; E9PB14; E9PBP7
• Molecular Weight: 50.4 kDa (471aa)
• NCBI Official Full Name: pyruvate dehydrogenase protein X component, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyruvate dehydrogenase complex component X
• NCBI Official Symbol: PDHX
• NCBI Official Synonym Symbols: E3BP; OPDX; PDX1; proX; DLDBP; PDHXD
• NCBI Protein Information: pyruvate dehydrogenase protein X component, mitochondrial
• UniProt Protein Name: Pyruvate dehydrogenase protein X component, mitochondrial
• Protein Family: Pyridoxal 5'-phosphate synthase
• UniProt Gene Name: PDHX
• UniProt Synonym Gene Names: PDX1; E3BP
• UniProt Entry Name: ODPX_HUMAN

NCBI Description

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]

Uniprot Description

PDHX: a mitochondrial protein required for anchoring dihydrolipoamide dehydrogenase (E3) to the pyruvate dehydrogenase complexes (PDHC) of eukaryotes. This specific binding is essential for a PDHC function. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and protein X bound by non-covalent bonds. Belongs to the 2-oxoacid dehydrogenase family. Contains 1 lipoyl-binding domain.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: mitochondrial matrix

Molecular Function: transferase activity, transferring acyl groups

Biological Process: cellular metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process

Disease: Pyruvate Dehydrogenase E3-binding Protein Deficiency