Western Blot (WB)
(PDS5A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).)
Rabbit anti-Human PDS5A Polyclonal Antibody | anti-PDS5A antibody
PDS5A, CT (PDS5A, KIAA0648, PDS5, Sister chromatid cohesion protein PDS5 homolog A, Cell proliferation-inducing gene 54 protein, Sister chromatid cohesion protein 112)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(PDS5A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).)
Western Blot (WB)
(PDS5A Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene binds to the cohesin complex and associates with chromatin through most of the cell cycle. The encoded protein may play a role in regulating sister chromatid cohesion during mitosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Uniprot Description
Function: Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Ref.8 Ref.17
Subunit structure: Interacts with the cohesin complex. Interacts with WAPAL (via FGF motifs) or CDCA5 (via the FGF motif); the interaction is direct, cohesin-dependent and competitive. Interacts with SMC3. Interacts with TP63. Ref.7 Ref.8 Ref.10 Ref.13 Ref.17 Ref.20
Subcellular location: Nucleus. Note: Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase. Ref.4 Ref.7
Tissue specificity: Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney. Ref.4
Developmental stage: Cell cycle-regulated with highest level in G2 phase. Ref.4
Miscellaneous: HeLa cells with a reduced level of PDS5A show a mild defect in sister chromatid cohesion. HeLa cells with a reduced level of RAD21 show reduced association of PDS5A with chromatin.
Sequence similarities: Belongs to the PDS5 family.Contains 1 HEAT repeat.
Sequence caution: The sequence AAI26226.1 differs from that shown. Reason: Erroneous initiation. The sequence AAM82347.1 differs from that shown. Reason: Probable cloning artifact.The sequence CAH18263.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on PDS5A
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Product Notes
The PDS5A pds5a (Catalog #AAA649303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDS5A, CT (PDS5A, KIAA0648, PDS5, Sister chromatid cohesion protein PDS5 homolog A, Cell proliferation-inducing gene 54 protein, Sister chromatid cohesion protein 112) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDS5A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PDS5A pds5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDS5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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