Western Blot (WB)
(Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates.)
Rabbit PDPK1 Polyclonal Antibody | anti-PDPK1 antibody
Anti-PDPK1 Antibody
Western Blot (WB)
(Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PDPK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PDPK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
Uniprot Description
PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; Protein kinase, AGC; AGC group; PKB family
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cell projection; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; focal adhesion; intracellular; membrane; nucleoplasm; plasma membrane
Molecular Function: 3-phosphoinositide-dependent protein kinase activity; ATP binding; insulin receptor binding; phospholipase activator activity; phospholipase binding; protein binding; protein kinase binding; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase B; blood coagulation; calcium-mediated signaling; cell migration; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; focal adhesion formation; hyperosmotic response; innate immune response; insulin receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of toll-like receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mast cell degranulation; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; synaptic transmission; T cell costimulation; T cell receptor signaling pathway; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway
Research Articles on PDPK1
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Product Notes
The PDPK1 pdpk1 (Catalog #AAA8243063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDPK1 Antibody reacts with Human, Mouse, Rat, Bovine, Pig, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's PDPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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