Rabbit anti-Human PDLIM1 Polyclonal Antibody | anti-PDLIM1 antibody

PDLIM1 (PDZ and LIM Domain Protein 1, C-terminal LIM Domain Protein 1, Elfin, LIM Domain Protein CLP-36, CLIM1, CLP36) (PE)

Cat. Num. AAA6388920

• Gene Names: PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDLIM1; Polyclonal Antibody; PDLIM1 (PDZ and LIM Domain Protein 1; C-terminal LIM Domain Protein 1; Elfin; LIM Domain Protein CLP-36; CLIM1; CLP36) (PE); anti-PDLIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDLIM1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PDLIM1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PDLIM1, aa1-329 (NP_066272.1).
• Immunogen Sequence: MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PDLIM1 rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human liver.)

Western Blot (WB)

(PDLIM1 rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in human pancreas.)

Western Blot (WB)

(PDLIM1 rabbit polyclonal antibody. Western Blot analysis of PDLIM1 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of PDLIM1 expression in transfected 293T cell line by PDLIM1 polyclonal antibody. Lane 1: PDLIM1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PDLIM1 antibody

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

Product Categories/Family for anti-PDLIM1 antibody

Antibodies; PDZ Domain

NCBI and Uniprot Product Information

• NCBI GI #: 13994151
• NCBI GeneID: 9124
• NCBI Accession #: NP_066272.1
• NCBI GenBank Nucleotide #: NM_020992.2
• UniProt Accession #: O00151; Q5VZH5; Q9BPZ9; B2RBS6
• Molecular Weight: 36,072 Da
• NCBI Official Full Name: PDZ and LIM domain protein 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 1
• NCBI Official Symbol: PDLIM1
• NCBI Official Synonym Symbols: CLIM1; CLP36; CLP-36; hCLIM1
• NCBI Protein Information: PDZ and LIM domain protein 1; elfin; LIM domain protein CLP-36; carboxyl terminal LIM domain protein 1
• UniProt Protein Name: PDZ and LIM domain protein 1
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM1
• UniProt Synonym Gene Names: CLIM1; CLP36
• UniProt Entry Name: PDLI1_HUMAN

NCBI Description

This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]

Uniprot Description

CLIM1: a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: transcription factor complex; cytoskeleton; focal adhesion; cytoplasm

Molecular Function: zinc ion binding; transcription coactivator activity

Biological Process: regulation of transcription, DNA-dependent; response to hypoxia; response to oxidative stress