Human Nidogen2 ELISA Kit | Nidogen2 elisa kit

Human Nidogen2 ELISA Kit PicoKine

Cat. Num. AAA128024

• Gene Names: NID2; NID-2
• Reactivity: Human
• Applications: ELISA
• Synonyms: Nidogen2; N/A; Human Nidogen2 ELISA Kit PicoKine; Nidogen2 elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <75pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: CHO; Immunogen sequence: L31-K1375
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for Nidogen2 elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
  O.D.	0.042	0.167	0.234	0.412	0.663	0.935	1.345	2.078

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	217	475	3201	191	346	3527
  Standard deviation	16.14	24.61	114.28	11.56	14.81	232.78
  CV(%)	7.4%	5.2%	3.6%	6.1%	4.3%	6.6%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	217	187	287	163	214	5.64	2.6%
  Sample 2	475	399	511	593	495	37.19	7.5%
  Sample 3	3201	4223	3220	4079	3681	237.04	6.4%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Standard Curve (Sample)

(Human Nidogen2 PicoKine ELISA Kit standard curve)

Related Product Information for Nidogen2 elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for Nidogen2 elisa kit

PicoKine ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 144953895
• NCBI GeneID: 22795
• NCBI Accession #: NP_031387.3
• NCBI GenBank Nucleotide #: NM_007361.3
• UniProt Accession #: Q14112; O43710; A8K6I7; B4DU19
• Molecular Weight: 140,866 Da
• NCBI Official Full Name: nidogen-2
• NCBI Official Synonym Full Names: nidogen 2
• NCBI Official Symbol: NID2
• NCBI Official Synonym Symbols: NID-2
• NCBI Protein Information: nidogen-2
• UniProt Protein Name: Nidogen-2
• UniProt Gene Name: NID2
• UniProt Synonym Gene Names: NID-2