Western Blot (WB)
(Western blot analysis of PDI using HepG2 whole cell lysates)
Rabbit PDI Polyclonal Antibody | anti-PDI antibody
PDI Antibody
Gene Names
PDIA2; PDI; PDA2; PDIP; PDIR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PDI; Polyclonal Antibody; PDI Antibody; Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDIA2; PDIA2_HUMAN; PDIp; PDIR; protein disulfide isomerase family A member 2; protein disulfide isomerase; pancreatic; protein disulfide isomerase-associated 2; Protein disulfide-isomerase A2; Rho GDP dissociation inhibitor gamma; anti-PDI antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PDI antibody detects endogenous levels of total PDI
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
525
Applicable Applications for anti-PDI antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human PDI
Subcellular Location
Endoplasmic Reticulum
Tissue Specificity
Highly expressed in pancreas (at protein level).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PDI using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis of PDI using HepG2 whole cell lysates)
Related Product Information for anti-PDI antibody
Function: Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Subunit Structure: Monomer; predominantly as monomer under reducing conditions. Homodimer; disulfide-linked. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Post-translational Modifications: The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.
Similarity: Belongs to the protein disulfide isomerase family.
Subunit Structure: Monomer; predominantly as monomer under reducing conditions. Homodimer; disulfide-linked. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.
Post-translational Modifications: The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated.
Similarity: Belongs to the protein disulfide isomerase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 61 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
protein disulfide-isomerase A2
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 2
NCBI Official Symbol
PDIA2
NCBI Official Synonym Symbols
PDI; PDA2; PDIP; PDIR
NCBI Protein Information
protein disulfide-isomerase A2
UniProt Protein Name
Protein disulfide-isomerase A2
UniProt Gene Name
PDIA2
UniProt Synonym Gene Names
PDIP; PDIp
NCBI Description
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels. [provided by RefSeq, Sep 2017]
Uniprot Description
Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
Research Articles on PDI
Similar Products
Product Notes
The PDI pdia2 (Catalog #AAA9608195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDI Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the PDI pdia2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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