Rabbit PDHK1 Polyclonal Antibody | anti-PDHK1 antibody

PDHK1(Phospho-Thr338) Antibody

Cat. Num. AAA9416261

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: By immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Synonyms: PDHK1; Polyclonal Antibody; PDHK1(Phospho-Thr338) Antibody; PDK1; PDH kinase 1; anti-PDHK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of PDHK1 only when phosphorylated at threonine 338.
• Purity/Purification: By immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-PDHK1 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:500~1:1000
• Target Species: Human
• Immunogen: Peptide sequence around phosphorylation site of threonine 338(Y-S-T(p)-A-P) derived from Human PDHK1.
• Immunogen Type: Peptide-KLH
• Modification: Phospho-Thr338
• Preparation and Storage: Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Related Product Information for anti-PDHK1 antibody

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Product Categories/Family for anti-PDHK1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 514052656
• NCBI GeneID: 5163
• NCBI Accession #: NP_001265478.1
• NCBI GenBank Nucleotide #: NM_001278549.1
• UniProt Accession #: Q15118; Q308M4; B2R6T1; B7Z937; D3DPD8; E9PD65
• Molecular Weight: 49kd
• NCBI Official Full Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase 1
• NCBI Official Symbol: PDK1
• NCBI Protein Information: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• UniProt Gene Name: PDK1
• UniProt Synonym Gene Names: PDHK1; PDH kinase 1
• UniProt Entry Name: PDK1_HUMAN

Uniprot Description

PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: ATYPICAL group; EC 2.7.11.2; Kinase, protein; Mitochondrial; PDHK family; Protein kinase, atypical

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: protein binding; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cell proliferation; induction of apoptosis by oxidative stress; regulation of acetyl-CoA biosynthetic process from pyruvate