Rabbit anti-Human PDHA2 Polyclonal Antibody | anti-PDHA2 antibody

PDHA2, ID (PDHA2, PDHAL, Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial, PDHE1-A type II) (MaxLight 490)

Cat. Num. AAA6332337

• Gene Names: PDHA2; PDHAL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PDHA2; Polyclonal Antibody; ID (PDHA2; PDHAL; Pyruvate dehydrogenase E1 component subunit alpha; testis-specific form; mitochondrial; PDHE1-A type II) (MaxLight 490); PDHE1-A type II; anti-PDHA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDHA2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-PDHA2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 287-314 in the Central region of human PDHA2. Swiss/UniProt Accession: P29803
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NCI-H460 cell line lysates (35ug/lane) usingMBS647925. This demonstrates MBS647925 detected the PDHA2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human testis tissue using MBS647925 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647925 for immunohistochemistry.)

Product Categories/Family for anti-PDHA2 antibody

Antibodies; Mitochondrial Proteins

References

1. Pinheiro, A., et al. Mol. Genet. Metab. 99(4):425-430(2010). 2. Olsen, J.V., et al. Cell 127(3):635-648(2006). 3. Caruso, M., et al. J. Biol. Chem. 276(48):45088-45097(2001). 4. Jacobia, S.J., et al. Arch. Biochem. Biophys. 395(1):121-128(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 4885543
• NCBI GeneID: 5161
• NCBI Accession #: NP_005381.1
• NCBI GenBank Nucleotide #: NM_005390.4
• UniProt Accession #: P29803; Q0VDI5; Q4VC02; Q6NXQ1; B2R9Q3
• Molecular Weight: 42,933 Da
• NCBI Official Full Name: pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• NCBI Official Synonym Full Names: pyruvate dehydrogenase (lipoamide) alpha 2
• NCBI Official Symbol: PDHA2
• NCBI Official Synonym Symbols: PDHAL
• NCBI Protein Information: pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• UniProt Protein Name: Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
• Protein Family: Pyruvate dehydrogenase E1 component
• UniProt Gene Name: PDHA2
• UniProt Synonym Gene Names: PDHAL
• UniProt Entry Name: ODPAT_HUMAN

Uniprot Description

PDHA2: a testis-specific mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Expressed in postmeiotic spermatogenic cells.

Protein type: Carbohydrate Metabolism - pyruvate; Oxidoreductase; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.4.1; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis

Chromosomal Location of Human Ortholog: 4q22-q23

Cellular Component: mitochondrial matrix; nucleus; pyruvate dehydrogenase complex

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) activity

Biological Process: acetyl-CoA biosynthetic process from pyruvate; glucose metabolic process; pyruvate metabolic process; tricarboxylic acid cycle