Western Blot (WB)
(All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit PDHA1 Polyclonal Antibody | anti-Q6P948 antibody
Zebrafish PDHA1 Antibody (Center)
Gene Names
pdha1a; pdha1; zgc:73271; zgc:86692; im:6895726; wu:fd18b01; wu:fo96f03
Reactivity
Zebrafish; Predicted Reactivity: Bovine, Human, Monkey, Mouse, Pig, Rat
Applications
Western Blot, ELISA
Synonyms
PDHA1; Polyclonal Antibody; Zebrafish PDHA1 Antibody (Center); PDHE1-A type I; Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; PHE1A; PDHA1;pdha1a;pdha1b; anti-Q6P948 antibody
Host
Rabbit
Reactivity
Zebrafish; Predicted Reactivity: Bovine, Human, Monkey, Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This Zebrafish PDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
393
Applicable Applications for anti-Q6P948 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:1000
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Q6P948 antibody
Description: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Product Categories/Family for anti-Q6P948 antibody
References
Glushakova, L.G., et al. Mol. Genet. Metab. 98 (3):289-299 (2009). Joao Silva, M., et al. Eur. J. Pediatr. 168 (1):17-22 (2009). Boichard, A., et al. Mol. Genet. Metab. 93 (3):323-330 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43741 Da
NCBI Official Full Name
pyruvate dehydrogenase E1 alpha 1
NCBI Official Synonym Full Names
pyruvate dehydrogenase (lipoamide) alpha 1a
NCBI Official Symbol
pdha1a
NCBI Official Synonym Symbols
pdha1; zgc:73271; zgc:86692; im:6895726; wu:fd18b01; wu:fo96f03
NCBI Protein Information
pyruvate dehydrogenase E1 alpha 1
UniProt Protein Name
Pyruvate dehydrogenase E1 component subunit alpha
UniProt Gene Name
pdha1a
UniProt Entry Name
Q6P948_DANRE
Uniprot Description
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.
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Product Notes
The Q6P948 pdha1a (Catalog #AAA9217075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Zebrafish PDHA1 Antibody (Center) reacts with Zebrafish; Predicted Reactivity: Bovine, Human, Monkey, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDHA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:1000. Researchers should empirically determine the suitability of the Q6P948 pdha1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDHA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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