Rabbit anti-Human PDGFB Polyclonal Antibody | anti-PDGFB antibody

PDGFB, CT (Platelet-derived Growth Factor Subunit B, PDGF Subunit B, Platelet-derived Growth Factor B Chain, Platelet-derived Growth Factor beta Polypeptide, PDGF-2, Proto-oncogene c-Sis, Becaplermin, PDGF2, SIS) (Biotin)

Cat. Num. AAA6493802

• Gene Names: PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PDGFB; Polyclonal Antibody; CT (Platelet-derived Growth Factor Subunit B; PDGF Subunit B; Platelet-derived Growth Factor B Chain; Platelet-derived Growth Factor beta Polypeptide; PDGF-2; Proto-oncogene c-Sis; Becaplermin; PDGF2; SIS) (Biotin); anti-PDGFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDGFB.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PDGFB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 212-241 in the C-terminal region of human PDGFB, UniProt Accession #P01127.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot asalysis in A375 cell lysate usingMBS627956.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of 293 cell using MBS627956 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-PDGFB antibody

Antibodies; Growth Factors; Cytokines

References

1. Muller, C., et al., J. Biol. Chem. 278(20):18330-18335 (2003). 2. Laprise, M.H., et al., Blood 100(10):3578-3587 (2002). 3. Maire, G., et al., Cancer Genet. Cytogenet. 134(2):156-161 (2002). 4. Andrae, J., et al., Biochem. Biophys. Res. Commun. 296(3):604-611 (2002). 5. Ehrlich, H.P., et al., Cytokines Cell Mol Ther 7(3):85-90 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 15451786
• NCBI GeneID: 5155
• NCBI Accession #: NP_148937
• NCBI GenBank Nucleotide #: NM_033016.3
• UniProt Accession #: P01127; P78431; Q15354; Q6FHE7; Q9UF23; G3XAG8
• Molecular Weight: 14.7kDa (130aa) confirmed by MALDI-TOF
• NCBI Official Full Name: platelet-derived growth factor subunit B isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor subunit B
• NCBI Official Symbol: PDGFB
• NCBI Official Synonym Symbols: SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2
• NCBI Protein Information: platelet-derived growth factor subunit B
• UniProt Protein Name: Platelet-derived growth factor subunit B
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFB
• UniProt Synonym Gene Names: PDGF2; SIS; PDGF subunit B
• UniProt Entry Name: PDGFB_HUMAN

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: Golgi membrane; extracellular space; cell surface; basolateral plasma membrane; endoplasmic reticulum lumen; cytoplasm; extracellular region

Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; superoxide-generating NADPH oxidase activator activity; chemoattractant activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cyclin-dependent protein kinase activity; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; positive regulation of fibroblast growth factor receptor signaling pathway; cell projection biogenesis; protein amino acid phosphorylation; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of fibroblast proliferation; positive chemotaxis; transforming growth factor beta receptor signaling pathway; cell growth; positive regulation of mitotic cell cycle, embryonic; response to drug; substrate-bound cell migration; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of chemotaxis; activation of protein kinase B; positive regulation of blood vessel endothelial cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; activation of protein kinase activity; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; actin cytoskeleton organization and biogenesis; negative regulation of transcription, DNA-dependent; embryonic placenta development; peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; response to estradiol stimulus; response to insulin stimulus; platelet degranulation; positive regulation of MAPKKK cascade; positive regulation of glomerular filtration; positive regulation of cell proliferation; response to wounding; hemopoiesis; DNA replication; negative regulation of cell migration; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of phosphoinositide 3-kinase activity; peptidyl-serine phosphorylation; response to hypoxia; innate immune response; blood coagulation; positive regulation of DNA replication; positive regulation of cell migration

Disease: Meningioma, Familial, Susceptibility To; Dermatofibrosarcoma Protuberans; Basal Ganglia Calcification, Idiopathic, 5; Basal Ganglia Calcification, Idiopathic, 1