Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
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Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
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Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
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Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine Eotaxin monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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aaa12672 porcine no cross reaction with other factors typical testing data standard curve for reference only aaa12672_sc elisa kit eotaxin chemokine c motif ligand 11 ccl11 scya11 1 eosinophil chemotactic protein small inducible cytokine subfamily a cys member 10,732 da a11 ccl11_human 4506827 np_002977.1 p51671 nm_002986.2 p50877 q92490 q92491 601156 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6 sensitivity up to 5 intra precision ligand11 scya111 to5
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
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The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
⇄⧉products_description => string (988) "Background: Eotaxin (also designated eotaxin-1) is a member of the C-C or ? ...
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Background: Eotaxin (also designated eotaxin-1) is a member of the C-C or ? family of chemokines which is characterized by a pair of adjacent cysteine residues. Eotaxin was first purified from the bronchoalveolar lavage fluid of guinea pigs challenged with an aerosol allergen, and serves as a potent chemoattractant for eosinophils. Eosinophilia is a prominent feature of several allergic conditions and is thought to be a central event in maladies such as bronchial asthma, dermatitis, conjunctivitis and possibly inflammatory bowel disease. The cognate eotaxin receptor has been identified. Originally described as mouse orphan receptor (MIP-1a receptor-like 2), CKR-3 has been shown to not only serve as the high affinity receptor for eotaxin, but also for RANTES and MCP-3. CKR-3 is expressed on the cell surface of primary eosinophils and does not bind to other members of the C-C or C-X-C family of chemokines. CKR-3 also serves as a co-receptor for a restricted subset of viruses.
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aaa14071 rabbit human polyclonal igg purified by epitope affinity purification supplied in sterile phosphate buffered saline ph7.2 containing antibody stabilizer elisa eia flow cytometry fc facs immunohistochemistry ihc immunoprecipitation ip western blot wb 0.01 0.1 ug ml 5 10 2 1 eotaxin anti c motif chemokine 11 ccl11 10,732 da eosinophil chemotactic protein small inducible cytokine a11 scya11 4506827 np_002977.1 nm_002986.2 p51671 p50877 q92490 q92491 u46573 mrna ml5 chemokine11
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat Eotaxin-3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa12680 rat no cross reaction with other factors typical testing data standard curve for reference only aaa12680_sc elisa kit eotaxin 3 chemokine c motif ligand 26 ccl26 imac tsc 1 mip 4a scya26 4alpha 4 alpha n1 cc thymic stroma small inducible cytokine a26 macrophage inflammatory protein subfamily a cys member 10,648 da unq216 pro242 ccl26_human 4586873 baa36704.1 q9y258 q52lv8 a0n0q5 604697 samples serum plasma or cell culture supernatant and organizations in the natural recombinant enos concentration assay type sandwich detection range 10 ng ml 0.156 sensitivity 0.06 intra precision eotaxin3 ligand26 tsc1 4alpha4 range10
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Samples||Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate!!Assay Type||Quantitative Competitive!!Sensitivity||0.1 ng/mL.
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Monkey Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit
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Intended Uses: This MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of SheepMBP. This ELISA kit for research use only!<br><br>Principle of the Assay: MBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MBP antibody and an MBP-HRP conjugate. The assay sample and buffer are incubated together with MBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP concentration since MBP from samples and MBP-HRP conjugate compete for the anti-MBP antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP from the sample, fewer sites are left to bind MBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve.
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
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aaa16777 monkey typical testing data standard curve for reference only aaa16777_sc elisa kit monocyte chemotactic protein 1 and activating factor mcp chemokine c motif ligand 2 ccl2 hc11 mcaf mcp1 scya2 gdcf smc cf hsmcr30 small inducible cytokine a2 secretory je chemoattractant subfamily a cys member homologous to mouse sig 11,025 da ccl2_human 545465 aab29926.1 p13500 q9udf3 b2r4v3 gene 607948 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate assay type quantitative competitive sensitivity 0.1 ng ml protein1 ligand2 sensitivity0.1
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This assay has high sensitivity and excellent specificity for detection of mouse MCP-2. No significant cross-reactivity or interference between mouse MCP-2 and analogues was observed.
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Mouse monocyte chemotactic protein 2; MCP-2 ELISA kit; HC14; MCP-2; MCP2; SCYA10; SCYA8; monocyte chemoattractant protein 2; monocyte chemotactic protein 2; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys); member 8 (monocyte che; chemokine (C-C motif) ligand 8
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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aaa15171 mouse this assay has high sensitivity and excellent specificity for detection of mcp 2 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15171_td elisa kit chemokine c motif ligand 8 monocyte chemotactic protein hc14 mcp2 scya10 scya8 chemoattractant small inducible cytokine a8 subfamily a cys member che ccl8 ab023418 1810063b20rik 11,017 da ccl8_mouse 10946818 np_067418.1 q9z121 nm_021443.3 samples serum plasma tissue homogenates type quantitative sandwich range 6.25 pg ml 400 < 1.56 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ligand8 ml400
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Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Competitive!!Detection Range||0.5-10ng/mL!!Sensitivity||0.1ng/mL
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Intended Uses||This MCP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig MCP-1. This ELISA kit for research use only, not for therapeutic applications!
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Guinea pig Monocyte chemotactic protein 1/monocyte chemotactic and activating factor ELISA Kit
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<b>Principle of the assay: </b>MCP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MCP-1 antibody and an MCP-1-HRP conjugate. The assay sample and buffer are incubated together with MCP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP-1 concentration since MCP-1 from samples and MCP-1-HRP conjugate compete for the anti-MCP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP-1 from the sample, fewer sites are left to bind MCP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP-1 concentration in each sample is interpolated from this standard curve.
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
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aaa16516 guinea pig typical testing data standard curve for reference only aaa16516_td elisa kit monocyte chemotactic protein 1 and activating factor mcp chemokine c motif ligand 2 ccl2 hc11 mcaf mcp1 scya2 gdcf smc cf hsmcr30 small inducible cytokine a2 secretory je chemoattractant subfamily a cys member homologous to mouse sig 11,025 da ccl2_human 545465 aab29926.1 p13500 q9udf3 b2r4v3 gene 607948 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate assay type competitive detection range 0.5 10ng ml sensitivity 0.1ng intended uses this is 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications! protein1 ligand2 range0.5 is1.5
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Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse Eotaxin-3 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
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aaa13042 mouse typical testing data standard curve for reference only aaa13042_sc elisa kit eotaxin 3 chemokine c motif ligand 26 ccl26 imac tsc 1 mip 4a scya26 4alpha 4 alpha n1 cc thymic stroma small inducible cytokine a26 macrophage inflammatory protein subfamily a cys member 10,648 da unq216 pro242 ccl26_human 4586873 baa36704.1 q9y258 q52lv8 a0n0q5 604697 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 1000 pg ml 15.6 sensitivity up to 5 intra precision eotaxin3 ligand26 tsc1 4alpha4 to5
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This assay has high sensitivity and excellent specificity for detection of IP-10. No significant cross-reactivity or interference between IP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IP-10 and all the analogues, therefore, cross reaction may still exist in some cases.
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1514) "Principle of the Assay: IP-10 ELISA kit applies the quantitative sandwich en...
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Principle of the Assay: IP-10 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IP-10. Standards or samples are then added to the microtiter plate wells and IP-10 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IP-10 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IP-10 are added to each well to "sandwich" the IP-10 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IP-10 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IP-10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa16275 rabbit typical testing data standard curve for reference only aaa16275_sc elisa kit chemokine interferon inducible protein 10 ip c x motif ligand cxcl10 c7 ip10 crg 2 inp10 ifi10 mob 1 scyb10 gip gamma activated gene small cytokine b10 induced kda b subfamily cys member 10,789 da crg2 cxl10_mouse 976159 aaa75249.1 p17515 immunology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type quantitative competitive sensitivity 1.0 pg ml protein10 mob1 sensitivity1.0
⇄⧉specificity => string (180) "The Rat CCL5 ELISA Kit allows for the detection and quantification of endoge...
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The Rat CCL5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CCL5 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
⇄purity => string (3) "N/A"
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⇄⧉storage_stability => string (107) "Shipped and store at 4 degree C for 6 months, store at -20 degree C for one ...
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Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
D17S136E; SCYA5; C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES
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⇄⧉products_description => string (1571) "Principle of the Assay: The Rat CCL5 ELISA (Enzyme-Linked Immunosorbent Assa...
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Principle of the Assay: The Rat CCL5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CCL5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.<br><br>Background/Introduction: Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4 (+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
C-C motif chemokine 5; SIS-delta; small inducible cytokine A5; small-inducible cytokine A5; T-cell-specific protein RANTES; regulated upon activation normal T-cell expressed and secreted
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aaa17858 rat the ccl5 elisa kit allows for detection and quantification of endogenous levels natural or recombinant proteins within range 15.6 pg ml 1000 sandwich se typical testing data standard curve reference only aaa17858_sc d17s136e scya5 c motif chemokine 5 eocp eosinophil chemotactic cytokine sis delta small inducible a5 t cell specific protein p228 tcp228 rantes ligand regulated upon activation normal expressed secreted 10,170 da ccl5_rat 1711393 p50231.1 p50231 samples culture supernatants serum plasma sensitivity < 1 chemokine5 <1
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CXCL17. No significant cross-reactivity or interference between CXCL17 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCL17 and all the analogues, therefore, cross reaction may still exist in some cases.
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Principle of the Assay: CXCL17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CXCL17 antibody and an CXCL17-HRP conjugate. The assay sample and buffer are incubated together with CXCL17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL17 concentration since CXCL17 from samples and CXCL17-HRP conjugate compete for the anti-CXCL17 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL17 from the sample, fewer sites are left to bind CXCL17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL17 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This CXCL17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CXCL17. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉ncbi_protein_info => string (197) "C-C motif chemokine 15; leukotactin 1; chemokine CC-2; new CC chemokine 3; s...
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C-C motif chemokine 15; leukotactin 1; chemokine CC-2; new CC chemokine 3; small-inducible cytokine A15; macrophage inflammatory protein 5; small inducible cytokine subfamily A (Cys-Cys), member 15
⇄⧉specificity => string (183) "This assay has high sensitivity and excellent specificity for detection of h...
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This assay has high sensitivity and excellent specificity for detection of human CCL16. No significant cross-reactivity or interference between human CCL16 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL16 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉ncbi_protein_info => string (293) "C-C motif chemokine 16; monotactin-1; chemokine LEC; chemokine CC-4; new CC ...
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C-C motif chemokine 16; monotactin-1; chemokine LEC; chemokine CC-4; new CC chemokine 4; liver CC chemokine-1; IL-10-inducible chemokine; liver-expressed chemokine; small-inducible cytokine A16; lymphocyte and monocyte chemoattractant; small inducible cytokine subfamily A (Cys-Cys), member 16
⇄⧉search_terms => string (639) "aaa18250 human this assay has high sensitivity and excellent specificity for...
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aaa18250 human this assay has high sensitivity and excellent specificity for detection of ccl16 no significant cross reactivity or interference between analogues was observed elisa kit chemokine c motif ligand 16 ckb12 hcc 4 ilinck lcc 1 lec lmc mgc117051 mtn ncc ncc4 scya16 scyl4 il 10 inducible cc liver expressed lymphocyte monotactin new small cytokine a16 monocyte chemoattractant subfamily a cys member 13,600 da ccl16_human 4759074 np_004581.1 o15467 nm_004590.2 q4kku0 601394 samples serum plasma tissue homogenates type sandwich range 39.06 pg ml 2500 typically less than 9.77 intra precision within an cv ligand16 hcc4 lcc1 il10
⇄⧉etc_term1 => string (158) "Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue hom...
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Samples||Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate!!Assay Type||Sandwich!!Detection Range||25-500pg/mL!!Sensitivity||1.0pg/mL
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Intended Uses||This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IP-10. This ELISA kit for research use only, not for therapeutic applications!
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⇄products_name => string (41) "Chemokine interferon-inducible protein 10"
⇄⧉products_description => string (1188) "<b>Principle of the assay: </b>IP-10 ELISA kit applies the competitive enzym...
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<b>Principle of the assay: </b>IP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IP-10 antibody and an IP-10-HRP conjugate. The assay sample and buffer are incubated together with IP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-10 concentration since IP-10 from samples and IP-10-HRP conjugate compete for the anti-IP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-10 from the sample, fewer sites are left to bind IP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (647) "aaa16970 canine typical testing data standard curve for reference only aaa16...
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aaa16970 canine typical testing data standard curve for reference only aaa16970_td elisa kit chemokine interferon inducible protein 10 ip c x motif ligand cxcl10 c7 ip10 crg 2 inp10 ifi10 mob 1 scyb10 gip gamma activated gene small cytokine b10 induced kda b subfamily cys member 10,789 da crg2 cxl10_mouse 976159 aaa75249.1 p17515 immunology samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type sandwich detection range 25 500pg ml sensitivity 1.0pg intended uses this is a 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications! protein10 mob1 range25 a1.5
⇄⧉testing_protocols => string (1122) "ICC (Immunocytochemistry)||ICC staining RANTES in HUVEC cells (green). The n...
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ICC (Immunocytochemistry)||ICC staining RANTES in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30380_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining RANTES in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30380_ICC5.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human lung caner tissue using anti-RANTES antibody. Counter stained with hematoxylin.||AAA30380_IHC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RANTES antibody. Counter stained with hematoxylin.||AAA30380_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RANTES antibody. Counter stained with hematoxylin.||AAA30380_IHC2.jpg!!WB (Western Blot)||Western blot analysis of RANTES on mouse thymus tissue lysate using anti-RANTES antibody at 1/1, 000 dilution.||AAA30380_WB.jpg
Beta chemokine RANTES antibody; Beta chemokine RANTES precursor antibody; C C motif chemokine 5 antibody; CCL 5 antibody; CCL5 antibody; CCL5_HUMAN antibody; Chemokine (C C motif) ligand 5 antibody; Chemokine CC Motif Ligand 5 antibody; D17S136E antibody; EoCP antibody; Eosinophil chemotactic cytokine antibody; MGC17164 antibody; RANTES (4-68) antibody; Regulated upon activation normally T expressed and presumably secreted antibody; SCYA 5 antibody; SCYA5 antibody; SIS delta antibody; SIS-delta antibody; SISd antibody; Small inducible cytokine A5 (RANTES) antibody; Small inducible cytokine A5 antibody; Small inducible cytokine subfamily A (Cys Cys) member 5 antibody; Small-inducible cytokine A5 antibody; T cell specific protein p288 antibody; T cell specific RANTES protein antibody; T cell-specific protein P228 antibody; T-cell-specific protein RANTES antibody; TCP 228 antibody; TCP228 antibody
⇄products_gene_name => string (6) "RANTES"
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⇄⧉products_description => string (747) "Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. ...
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Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES (3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES (4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES (1-68) and RANTES (3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
⇄⧉ncbi_protein_info => string (239) "C-C motif chemokine 5; beta-chemokine RANTES; T-cell specific protein p288; ...
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C-C motif chemokine 5; beta-chemokine RANTES; T-cell specific protein p288; eosinophil chemotactic cytokine; small inducible cytokine subfamily A (Cys-Cys), member 5; regulated upon activation, normally T-expressed, and presumably secreted
aaa30380 rabbit human mouse monoclonal jm03 45 proa affinity purified 1*tbs ph7.4 1 bsa 40 glycerol preservative 0.05 sodium azide western blot wb immunocytochemistry icc immunofluorescence if immunohistochemistry ihc 1:500 1:1000 1:50 1:200 analysis of rantes on thymus tissue lysate using anti antibody at 000 dilution aaa30380_wb immunohistochemical paraffin embedded tonsil counter stained with hematoxylin aaa30380_ihc2 breast cancer aaa30380_ihc3 lung caner aaa30380_ihc4 staining in hela cells green the nuclear stain is dapi blue were fixed paraformaldehyde permeabilised 0.25 triton x100 pbs aaa30380_icc5 huvec aaa30380_icc6 beta chemokine precursor c motif 5 ccl ccl5 ccl5_human ligand cc d17s136e eocp eosinophil chemotactic cytokine mgc17164 4 68 regulated upon activation normally t expressed and presumably secreted scya scya5 sis delta sisd small inducible a5 subfamily a cys member cell specific protein p288 p228 tcp 228 tcp228 9,990 da 2905632 aac03541.1 p13501 o43646 q0qvw8 q4zgj1 q9nya2 q9ubg2 q9uc99 187011 total ab type recombinant immunogen conjugation unconjugated jm0345 ph7.41 bsa40 at000 motif5 mgc171644
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of C...
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This assay has high sensitivity and excellent specificity for detection of CXCL-10. No significant cross-reactivity or interference between CXCL-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CXCL-10 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1429) "Principle of the Assay: CXCL-10 ELISA kit applies the competitive enzyme imm...
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Principle of the Assay: CXCL-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CXCL-10 antibody and an CXCL-10-HRP conjugate. The assay sample and buffer are incubated together with CXCL-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCL-10 concentration since CXCL-10 from samples and CXCL-10-HRP conjugate compete for the anti-CXCL-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCL-10 from the sample, fewer sites are left to bind CXCL-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL-10 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This CXCL-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CXCL-10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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aaa17080 monkey typical testing data standard curve for reference only aaa17080_td elisa kit cxc chemokine ligand 10 cxcl10 c x motif c7 ifi10 inp10 ip crg 2 mob 1 scyb10 gip gamma ip10 small inducible cytokine b10 interferon kda induced protein from cell line subfamily b cys member 10,881 da b10cleaved into the following chain:cxcl10 73 cxl10_human 149999382 np_001556.2 p02778 nm_001565.3 q96qj5 147310 immunology samples serum plasma culture supernatants body fluid and tissue homogenate assay type competitive detection range 0.5 10ng ml sensitivity 0.1ng ligand10 crg2 mob1 chain:cxcl1073 range0.5
⇄⧉specificity => string (185) "This assay has high sensitivity and excellent specificity for detection of m...
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This assay has high sensitivity and excellent specificity for detection of monkey CCL17. No significant cross-reactivity or interference between monkey CCL17 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Monkey C-C motif chemokine 17 (CCL17) ELISA kit; A-152E5.3; ABCD-2; MGC138271; MGC138273; SCYA17; TARC; T cell-directed CC chemokine; small inducible cytokine A17; small inducible cytokine subfamily A (Cys-Cys); member 17; thymus and activation-regul; chemokine (C-C motif) ligand 17
⇄products_gene_name => string (5) "CCL17"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (735) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (737) "aaa18087 monkey this assay has high sensitivity and excellent specificity fo...
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aaa18087 monkey this assay has high sensitivity and excellent specificity for detection of ccl17 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa18087_td elisa kit chemokine c motif ligand 17 a 152e5.3 abcd 2 mgc138271 mgc138273 scya17 tarc t cell directed cc small inducible cytokine a17 subfamily cys member thymus activation regul regulated 10,549 da ccl17_macmu 74136265 np_001028024.1 q8hyp9 nm_001032852.1 samples serum plasma tissue homogenates type quantitative sandwich range 39 pg ml 2500 <9.8 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ligand17 abcd2 range39
⇄⧉specificity => string (179) "This assay has high sensitivity and excellent specificity for detection of r...
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This assay has high sensitivity and excellent specificity for detection of rat IP-10. No significant cross-reactivity or interference between rat IP-10 and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
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Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Rat interferon-inducible protein 10; IP-10 ELISA Kit; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1; gamma IP10; interferon-inducible cytokine IP-10; protein 10 from interferon (gamma) -induced cell line; small inducible cytokine B10; small inducibl; chemokine (C-X-C motif) ligand 10
⇄products_gene_name => string (6) "CXCL10"
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⇄products_gene_name_syn => string (3) "N/A"
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⇄⧉products_description => string (735) "Principle of the Assay||This assay employs the quantitative sandwich enzyme ...
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Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IP-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IP-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IP-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IP-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
C-X-C motif chemokine 10; gamma-IP10; protein Mob-1; small-inducible cytokine B10; interferon-inducible protein 10; interferon-inducible cytokine IP-10; 10 kDa interferon gamma-induced protein; small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10
⇄⧉search_terms => string (728) "aaa15325 rat this assay has high sensitivity and excellent specificity for d...
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aaa15325 rat this assay has high sensitivity and excellent specificity for detection of ip 10 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15325_td elisa kit chemokine c x motif ligand interferon inducible protein c7 ifi10 inp10 scyb10 crg 2 gip mob 1 gamma ip10 cytokine from induced cell line small b10 inducibl cxcl10 kda b subfamily cys member 10,729 da mob1 cxl10_rat 20806125 np_620789.1 p48973 nm_139089.1 p70538 q6gtc7 samples serum plasma tissue homogenates type sandwich range 781.25 pg ml 50000 195 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in crg2
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of C...
$value[16]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of CX3CL1. No significant cross-reactivity or interference between CX3CL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CX3CL1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[16]['_source']['purity']
⇄form => string (3) "N/A"
$value[16]['_source']['form']
⇄concentration => string (3) "N/A"
$value[16]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1409) "Intended Uses: This CX3CL1 ELISA kit is a 1.5 hour solid-phase ELISA designe...
$value[16]['_source']['products_description']
Intended Uses: This CX3CL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MonkeyCX3CL1. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: CX3CL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CX3CL1 antibody and an CX3CL1-HRP conjugate. The assay sample and buffer are incubated together with CX3CL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX3CL1 concentration since CX3CL1 from samples and CX3CL1-HRP conjugate compete for the anti-CX3CL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX3CL1 from the sample, fewer sites are left to bind CX3CL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX3CL1 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (768) "aaa16851 monkey this assay has high sensitivity and excellent specificity fo...
$value[16]['_source']['search_terms']
aaa16851 monkey this assay has high sensitivity and excellent specificity for detection of cx3cl1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16851_sc elisa kit fractalkine chemokine c x3 motif ligand 1 ntn ntt cxc3 cxc3c scyd1 abcd 3 c3xkine neurotactin small inducible cytokine d1 cx3c membrane anchored subfamily d cys member 42,187 da protein q6i9s9_human 48146969 cag33707.1 p78423 q6i9s9 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 0.1 ng ml ligand1 abcd3 competitive0.1
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of I...
$value[17]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of IP-10. No significant cross-reactivity or interference between IP-10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IP-10 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[17]['_source']['purity']
⇄form => string (3) "N/A"
$value[17]['_source']['form']
⇄concentration => string (3) "N/A"
$value[17]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1398) "Intended Uses: This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed...
$value[17]['_source']['products_description']
Intended Uses: This IP-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IP-10. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: IP-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IP-10 antibody and an IP-10-HRP conjugate. The assay sample and buffer are incubated together with IP-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IP-10 concentration since IP-10 from samples and IP-10-HRP conjugate compete for the anti-IP-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by IP-10 from the sample, fewer sites are left to bind IP-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IP-10 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (759) "aaa16919 porcine this assay has high sensitivity and excellent specificity f...
$value[17]['_source']['search_terms']
aaa16919 porcine this assay has high sensitivity and excellent specificity for detection of ip 10 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16919_sc elisa kit chemokine interferon inducible protein c x motif ligand cxcl10 c7 ip10 crg 2 inp10 ifi10 mob 1 scyb10 gip gamma activated gene small cytokine b10 induced kda b subfamily cys member 10,789 da crg2 cxl10_mouse 976159 aaa75249.1 p17515 immunology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml mob1 competitive1.0
⇄⧉specificity => string (169) "This assay has high sensitivity and excellent specificity for detection of I...
$value[18]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of IP10. No significant cross-reactivity or interference between IP10 and analogues was observed.
⇄purity => string (3) "N/A"
$value[18]['_source']['purity']
⇄form => string (3) "N/A"
$value[18]['_source']['form']
⇄concentration => string (3) "N/A"
$value[18]['_source']['concentration']
⇄⧉storage_stability => string (474) "The stability of kit is determined by the loss rate of activity. The loss ra...
$value[18]['_source']['storage_stability']
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.<br>To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Samples||Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids!!Assay Type||Quantitative Sandwich!!Detection Range||15.6-1,000pg/mL!!Sensitivity||< 6.0pg/mL
⇄⧉etc_term2 => string (414) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 ...
$value[18]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IP10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%!!Inter-assay Precision||Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IP10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
⇄products_price => string (6) "0.0000"
$value[18]['_source']['products_price']
⇄products_weight => string (4) "5.00"
$value[18]['_source']['products_weight']
⇄products_status => boolean true
$value[18]['_source']['products_status']
⇄products_tax_class_id => string (1) "1"
$value[18]['_source']['products_tax_class_id']
⇄manufacturers_id => string (4) "2700"
$value[18]['_source']['manufacturers_id']
⇄products_ordered => string (1) "0"
$value[18]['_source']['products_ordered']
⇄language_id => string (1) "1"
$value[18]['_source']['language_id']
⇄products_name => string (45) "Interferon Gamma Induced Protein 10kDa (IP10)"
⇄⧉products_description => string (1023) "Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantit...
$value[18]['_source']['products_description']
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IP10 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.<br><br>Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IP10. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IP10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄⧉search_terms => string (803) "aaa22926 human this assay has high sensitivity and excellent specificity for...
$value[18]['_source']['search_terms']
aaa22926 human this assay has high sensitivity and excellent specificity for detection of ip10 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa22926_sc elisa kit interferon gamma induced protein 10kda cxcl10 c7 ifi10 inp10 scyb10 crg2 gip10 mob1 chemokine c x motif ligand 10 small inducible cytokine subfamily b cys member 11,329 da 535219 caa84455.1 q38382 samples serum plasma tissue homogenates cell lysates culture supernates other biological fluids type quantitative sandwich range 15.6 1,000pg ml < 6.0pg intra precision within an 3 with low middle level were tested 20 times on one plate respectively cv<10 inter assays different plates 8 replicates in each cv = sd meanx100 cv<12 ligand10 an3 tested20 plates8
⇄purity => string (69) "CCL2 Antibody is affinity chromatography purified via peptide column."
$value[19]['_source']['purity']
⇄form => string (71) "Liquid; CCL2 Antibody is supplied in PBS containing 0.02% sodium azide."
$value[19]['_source']['form']
⇄concentration => string (7) "1 mg/mL"
$value[19]['_source']['concentration']
⇄⧉storage_stability => string (103) "CCL2 antibody can be stored at 4 degree C for three months and -20 degree C,...
$value[19]['_source']['storage_stability']
CCL2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
⇄⧉app_tested => string (94) "ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immun...
$value[19]['_source']['app_tested']
ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-P), Immunofluorescence (IF)
⇄⧉app_notes => string (344) "CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 ug/...
$value[19]['_source']['app_notes']
CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 ug/mL.<br>Optimal dilutions for each application to be determined by the researcher.<br>Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples<br>and Immunofluorescence in human samples. All other applications and species not yet tested.
⇄⧉testing_protocols => string (388) "IF (Immunofluorescence)||Immunofluorescence of CCL2 in human spleen tissue w...
$value[19]['_source']['testing_protocols']
IF (Immunofluorescence)||Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.||AAA10976_IF3.jpg!!IHC (Immunohistochemistry)||Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.||AAA10976_IHC2.jpg!!WB (Western Blot)||Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.||AAA10976_WB.jpg
⇄⧉etc_term1 => string (219) "Conjugate||Unconjugated!!Immunogen||Rabbit polyclonal CCL2 antibody was rais...
$value[19]['_source']['etc_term1']
Conjugate||Unconjugated!!Immunogen||Rabbit polyclonal CCL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human CCL2.<br>The immunogen is located within the last 50 amino acids of CCL2.
⇄⧉etc_term2 => string (141) "Homology||Predicted species reactivity based on immunogen sequence: Pig: (81...
$value[19]['_source']['etc_term2']
Homology||Predicted species reactivity based on immunogen sequence: Pig: (81%), Horse: (81%), Bovine: (75%), Rabbit: (75%), Guinea pig: (90%)
⇄⧉products_description => string (682) "CCL2 Antibody: CCL2, also known as monocyte chemotactic protein 1 (MCP1), be...
$value[19]['_source']['products_description']
CCL2 Antibody: CCL2, also known as monocyte chemotactic protein 1 (MCP1), belongs to the intercrine beta (chemokine CC) family. It is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. It is upregulated during infection and inflammation. CCL2 is a potent basophil activator but does not affect eosinophils, whereas the related protein MCP2 stimulates both eosinophils and basophils. MCP3 has been shown to have the broadest range of influence. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
⇄⧉search_terms => string (1142) "aaa10976 rabbit human mouse rat polyclonal igg ccl2 antibody is affinity chr...
$value[19]['_source']['search_terms']
aaa10976 rabbit human mouse rat polyclonal igg ccl2 antibody is affinity chromatography purified via peptide column liquid supplied in pbs containing 0.02 sodium azide elisa eia western blot wb immunohistochemistry paraffin ihc p immunofluorescence if can be used for detection of by at 1 2 ug ml optimal dilutions each application to determined the researcher validated samples and all other applications species not yet tested postive control mbs151739 k562 cell lysate mbs154333 spleen slide analysis with μ g aaa10976_wb4 tissue 5 aaa10976_ihc2 20 aaa10976_if3 hc11 mcaf mcp1 mcp scya2 gdcf smc cf hsmcr30 c motif chemokine ligand small inducible cytokine a2 monocyte secretory protein je chemotactic chemoattractant activating factor subfamily a cys member homologous sig predicted 11kda observed 14kda ccl2_human 4506841 np_002973 p13500 nm_002982.3 q9udf3 b2r4v3 gene 607948 conjugate unconjugated immunogen was raised against 17 amino acid near carboxy terminus located within last 50 acids homology reactivity based on sequence pig 81 horse bovine 75 guinea 90 at1 tissue5 aaa10976_ihc220 against17 last50 pig81 bovine75 guinea90
