Rabbit anti-Human, Mouse PDE6C Polyclonal Antibody | anti-PDE6C antibody

PDE6C, ID (PDE6C, PDEA2, Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha', cGMP phosphodiesterase 6C) (MaxLight 490)

Cat. Num. AAA6332172

• Gene Names: PDE6C; COD4; ACHM5; PDEA2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography followed by Peptide Affinity Purification.
• Synonyms: PDE6C; Polyclonal Antibody; ID (PDE6C; PDEA2; Cone cGMP-specific 3'; 5'-cyclic phosphodiesterase subunit alpha'; cGMP phosphodiesterase 6C) (MaxLight 490); cGMP phosphodiesterase 6C; anti-PDE6C antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDE6C. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography followed by Peptide Affinity Purification.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-PDE6C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within aa271-300 in central region of of human PDE6C, UniProt accession #P51160; Q91ZQ1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PDE6C using MBS641996 in mouse liver tissue lysate (35ug/lane).)

Immunohistochemistry (IHC)

(Immunohistochemistry staining of formalin-fixed and paraffin-embedded human testis usingMBS641996. Peroxidase-conjugated secondary antibody/DAB staining were used for visualization.)

Related Product Information for anti-PDE6C antibody

PDE6C is the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa.

Product Categories/Family for anti-PDE6C antibody

Antibodies; Enzymes; Phosphodiesterase

References

1. Muradov, H., et al. J. Biol. Chem. 284(47): 32662-32669 (2009). 2. Chang, B., et al. Proc. Natl. Acad. Sci. U.S.A. 106(46):19581-19586(2009). 3. Thiadens, A.A., et al. Am. J. Hum. Genet. 85(2):240-247 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 109939748
• NCBI GeneID: 5146
• UniProt Accession #: P51160; Q91ZQ1
• Molecular Weight: ~125 kDa
• NCBI Official Full Name: Synthetic construct Homo sapiens clone IMAGE:40080654, MGC:133442 PDE6C protein (PDE6C) mRNA, encodes complete protein
• NCBI Official Synonym Full Names: phosphodiesterase 6C, cGMP-specific, cone, alpha prime
• NCBI Official Symbol: PDE6C
• NCBI Official Synonym Symbols: COD4; ACHM5; PDEA2
• NCBI Protein Information: cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'; cGMP phosphodiesterase 6C
• UniProt Protein Name: Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'
• Protein Family: Cone cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6C
• UniProt Synonym Gene Names: PDEA2
• UniProt Entry Name: PDE6C_HUMAN

NCBI Description

This gene encodes the alpha-prime subunit of cone phosphodiesterase, which is composed of a homodimer of two alpha-prime subunits and 3 smaller proteins of 11, 13, and 15 kDa. Mutations in this gene are associated with cone dystrophy type 4 (COD4). [provided by RefSeq, Mar 2010]

Uniprot Description

Catalytic activity: Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactor: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions

By similarity.

Subunit structure: Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential.

Involvement in disease: Cone dystrophy 4 (COD4) [MIM:613093]: An early-onset cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7

Sequence similarities: Belongs to the cyclic nucleotide phosphodiesterase family.Contains 2 GAF domains.