Rabbit anti-Human, Mouse PDE4A Polyclonal Antibody | anti-PDE4A antibody

PDE4A, NT (PDE4A, DPDE2, cAMP-specific 3',5'-cyclic phosphodiesterase 4A, PDE46) (FITC)

Cat. Num. AAA6332125

• Gene Names: PDE4A; PDE4; DPDE2; PDE46
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE4A; Polyclonal Antibody; NT (PDE4A; DPDE2; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4A; PDE46) (FITC); PDE46; anti-PDE4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 860

• Applicable Applications for anti-PDE4A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human PDE4A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE4A Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the PDE4A antibody detected the PDE4A protein (arrow).)

Related Product Information for anti-PDE4A antibody

Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE (summary by Milatovich et al., 1994 [PubMed 8009369]).

Product Categories/Family for anti-PDE4A antibody

Antibodies; Enzymes; Phosphodiesterase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Selige, J., et al. J. Cell. Physiol. 223(2):317-326(2010). Bjorgo, E., et al. Mol. Cell. Biol. 30(7):1660-1672(2010). Singh, D., et al. Respir. Res. 11, 26 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 162329610
• NCBI GeneID: 5141
• NCBI Accession #: NP_001104778.1; NP_001104777.1
• NCBI GenBank Nucleotide #: NP_001104778.1
• UniProt Accession #: P27815
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4A isoform 2
• NCBI Official Synonym Full Names: phosphodiesterase 4A
• NCBI Official Symbol: PDE4A
• NCBI Official Synonym Symbols: PDE4; DPDE2; PDE46
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4A
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4A
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE4A
• UniProt Synonym Gene Names: DPDE2
• UniProt Entry Name: PDE4A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

PDE4A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum. Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4-[(3-butoxy-4-methoxyphenyl)-methyl]- 2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; EC 3.1.4.53; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: membrane; perinuclear region of cytoplasm; cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; metal ion binding; cAMP binding

Biological Process: sensory perception of smell; cAMP catabolic process; signal transduction