Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: PDE1B antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62, 60 kDaObserved band size: 62 kDa)
Rabbit anti-Human PDE1B Polyclonal Antibody | anti-PDE1B antibody
PDE1B Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IF: 1:50-1:200
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: PDE1B antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62, 60 kDaObserved band size: 62 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: PDE1B antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62, 60 kDaObserved band size: 62 kDa)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF-7 with MBS7134027 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF-7 with MBS7134027 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
PDE1B: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase; Apoptosis
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: cell soma; cytosol
Molecular Function: calmodulin binding; 3',5'-cyclic-AMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; metal ion binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; monocyte differentiation; apoptosis; regulation of neurotransmitter levels; response to amphetamine; cAMP catabolic process; regulation of dopamine metabolic process; locomotory behavior; signal transduction; phospholipase C activation; innate immune response; visual learning; cGMP catabolic process; blood coagulation; serotonin metabolic process
Research Articles on PDE1B
Similar Products
Product Notes
The PDE1B pde1b (Catalog #AAA7134027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE1B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the PDE1B pde1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
