Rabbit PDCD4 Polyclonal Antibody | anti-PDCD4 antibody

RABBIT ANTI HUMAN PDCD4 (C-TERMINAL)

Cat. Num. AAA222849

• Gene Names: PDCD4; H731
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: PDCD4; Polyclonal Antibody; RABBIT ANTI HUMAN PDCD4 (C-TERMINAL); anti-PDCD4 antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence), Xenopus (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. ; ; Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 469

• Applicable Applications for anti-PDCD4 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 52kDa.; ELISA: Minimum Dilution: 1/10000; Maximum Dilution: 1/50000; Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/300; Application Note: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to Pdcd4 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-PDCD4 antibody

MBS222849 detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor. Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.

NCBI and Uniprot Product Information

• NCBI GI #: 21735596
• NCBI GeneID: 27250
• NCBI Accession #: NP_055271.2
• NCBI GenBank Nucleotide #: NM_014456.4
• UniProt Accession #: Q53EL6; O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91
• Molecular Weight: 50,576 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 1
• NCBI Official Synonym Full Names: programmed cell death 4 (neoplastic transformation inhibitor)
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4; neoplastic transformation inhibitor protein; nuclear antigen H731; protein 197/15a
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731
• UniProt Entry Name: PDCD4_HUMAN

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle