Rabbit PCSK9 Polyclonal Antibody | anti-PCSK9 antibody

PCSK9 Rabbit Polyclonal

Cat. Num. AAA7602460

• Gene Names: PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PCSK9; Polyclonal Antibody; PCSK9 Rabbit Polyclonal; anti-PCSK9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 692

• Applicable Applications for anti-PCSK9 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Proprotein convertase subtilisin/kexin type 9
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using MBS7602460 (PCSK9 Antibody) at dilution of 1:100 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)

Western Blot (WB)

(COLO 320 cells were subjected to SDS PAGE followed by western blot with MBS7602460 (PCSK9 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 31317307
• NCBI GeneID: 255738
• NCBI Accession #: NP_777596.2
• NCBI GenBank Nucleotide #: NM_174936.3
• Molecular Weight: 20,827 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: PCSK9
• NCBI Official Synonym Symbols: FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 9
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK9
• UniProt Synonym Gene Names: NARC1; NARC-1; PC9
• UniProt Entry Name: PCSK9_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; Cell development/differentiation; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: cell surface; cytoplasm; early endosome; endoplasmic reticulum; ER to Golgi transport vesicle; extracellular space; extrinsic to external side of plasma membrane; Golgi apparatus; late endosome; lysosome; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum

Molecular Function: apolipoprotein binding; apolipoprotein receptor binding; low-density lipoprotein binding; low-density lipoprotein receptor binding; protein binding; protein self-association; receptor inhibitor activity; serine-type endopeptidase activity; sodium channel inhibitor activity; very-low-density lipoprotein binding

Biological Process: apoptosis; cellular response to insulin stimulus; cellular response to starvation; cholesterol homeostasis; cholesterol metabolic process; kidney development; lipoprotein metabolic process; liver development; low-density lipoprotein receptor catabolic process; lysosomal transport; negative regulation of receptor recycling; neurogenesis; neuron differentiation; phospholipid metabolic process; positive regulation of low-density lipoprotein receptor catabolic process; positive regulation of neuron apoptosis; positive regulation of receptor internalization; protein autoprocessing; regulation of neuron apoptosis; regulation of receptor activity; triacylglycerol metabolic process

Disease: Hypercholesterolemia, Autosomal Dominant, 3