Western Blot (WB)
(Western Blot analysis of KIAA0101 expression in transfected 293T cell line by KIAA0101 polyclonal antibody. Lane 1: KIAA0101 transfected lysate (12.32kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human PCNA-associated Factor Polyclonal Antibody | anti-2810417H13Rik antibody
PCNA-associated Factor (Hepatitis C Virus NS5A-transactivated Protein 9, HCV NS5A-transactivated Protein 9, Overexpressed in Anaplastic Thyroid Carcinoma 1, OEATC-1, PCNA-associated Factor of 15 kDa, PAF15, p15PAF, NS5ATP9, PAF, L5)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of KIAA0101 expression in transfected 293T cell line by KIAA0101 polyclonal antibody. Lane 1: KIAA0101 transfected lysate (12.32kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of KIAA0101 expression in transfected 293T cell line by KIAA0101 polyclonal antibody. Lane 1: KIAA0101 transfected lysate (12.32kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
Uniprot Description
Function: PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number
By similarity.
Subunit structure: Interacts (when monoubiquitinated at Lys-15 and Lys-24) with PCNA. Interacts with isoform 2/p33ING1b of ING1. Interacts with BRCA1
By similarity.
Subcellular location: Nucleus. Cytoplasm › perinuclear region
By similarity. Note: Following DNA damage, localizes to DNA damage sites. Colocalizes with centrosomes in perinuclear region.
Domain: The PIP-box mediates the interaction with PCNA
By similarity.The KEN box is required for the association with the APC/C complex
By similarity.The D-box (destruction box) mediates the interaction with APC/C proteins, and acts as a recognition signal for degradation via the ubiquitin-proteasome pathway
By similarity.The initiation motif is required for efficient chain initiation by the APC/C complex E2 ligase UBE2C. It determines the rate of substrate's degradation without affecting its affinity for the APC/C, a mechanism used by the APC/C to control the timing of substrate proteolysis during the cell cycle
By similarity.
Post-translational modification: Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome
By similarity.
Research Articles on 2810417H13Rik
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Product Notes
The 2810417H13Rik paf (Catalog #AAA648359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PCNA-associated Factor (Hepatitis C Virus NS5A-transactivated Protein 9, HCV NS5A-transactivated Protein 9, Overexpressed in Anaplastic Thyroid Carcinoma 1, OEATC-1, PCNA-associated Factor of 15 kDa, PAF15, p15PAF, NS5ATP9, PAF, L5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA-associated Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the 2810417H13Rik paf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVRTKADSVP GTYRKVVAAR APRKVLGSST SATNSTSVSS RKAENKYAGG NPVCVRPTPK WQKGIGEFFR LSPKDSEKEN QIPEEAGSSG LGKAKRKACP LQPD. It is sometimes possible for the material contained within the vial of "PCNA-associated Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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