Western Blot (WB)
(Biotinylated (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat PCNA Polyclonal Antibody | anti-PCNA antibody
Goat anti-PCNA, Biotinylated Antibody
Western Blot: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to NP_002583.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
(Biotinylated (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Uniprot Description
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.
Protein type: DNA replication; Cell cycle regulation
Chromosomal Location of Human Ortholog: 20pter-p12
Cellular Component: centrosome; cytoplasm; DNA replication factor C complex; nuclear chromosome, telomeric region; nuclear replication fork; nucleoplasm; nucleus; PCNA complex
Molecular Function: chromatin binding; damaged DNA binding; dinucleotide insertion or deletion binding; DNA polymerase processivity factor activity; enzyme binding; estrogen receptor binding; histone acetyltransferase binding; identical protein binding; MutLalpha complex binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding
Biological Process: bypass DNA synthesis; cell proliferation; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA strand elongation during DNA replication; epithelial cell differentiation; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; heart development; leading strand elongation; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of deoxyribonuclease activity; positive regulation of DNA repair; positive regulation of DNA replication; protein sumoylation; replication fork processing; response to cadmium ion; response to lipid; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
Disease: Ataxia-telangiectasia-like Disorder 2
Research Articles on PCNA
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Product Notes
The PCNA pcna (Catalog #AAA423608) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PCNA, Biotinylated Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 30kDa band observed in lysates of cell line HeLa (calculated MW of 28.8kDa according to NP_002583.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NGNIKLSQTS NVD. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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