Rabbit anti-Human PCDHB15 Polyclonal Antibody | anti-PCDHB15 antibody

PCDHB15, NT (PCDHB15, Protocadherin beta-15) (Biotin)

Cat. Num. AAA6331618

• Gene Names: PCDHB15; PCDH-BETA15
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHB15; Polyclonal Antibody; NT (PCDHB15; Protocadherin beta-15) (Biotin); Protocadherin beta-15; anti-PCDHB15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PCDHB15 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PCDHB15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-148 amino acids from the N-terminal region of human PCDHB15.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHB15 Antibody (N-term) western blot analysis in MDA-MB453,Jurkat,ZR-75-1,HL-60 cell line lysates (35ug/lane).This demonstrates the PCDHB15 antibody detected the PCDHB15 protein (arrow).)

Related Product Information for anti-PCDHB15 antibody

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.

Product Categories/Family for anti-PCDHB15 antibody

Antibodies; Cadherin

References

Vanhalst, K., et al. FEBS Lett. 495 (1-2), 120-125 (2001): Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 9256610
• NCBI GeneID: 56121
• NCBI Accession #: NP_061758.1
• NCBI GenBank Nucleotide #: NM_018935.2
• UniProt Accession #: Q9Y5E8; Q8IUX5
• Molecular Weight: 86,329 Da
• NCBI Official Full Name: protocadherin beta-15
• NCBI Official Synonym Full Names: protocadherin beta 15
• NCBI Official Symbol: PCDHB15
• NCBI Official Synonym Symbols: PCDH-BETA15
• NCBI Protein Information: protocadherin beta-15; PCDH-beta-15; OTTHUMP00000160009
• UniProt Protein Name: Protocadherin beta-15
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHB15
• UniProt Entry Name: PCDBF_HUMAN

NCBI Description

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq]

Uniprot Description

PCDHB15: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to plasma membrane

Molecular Function: calcium ion binding

Biological Process: nervous system development; cell adhesion; homophilic cell adhesion