Rabbit anti-Human PCDHB14 Polyclonal Antibody | anti-PCDHB14 antibody

PCDHB14, NT (PCDHB14, Protocadherin beta-14) (Biotin)

Cat. Num. AAA6331607

• Gene Names: PCDHB14; PCDH-BETA14
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHB14; Polyclonal Antibody; NT (PCDHB14; Protocadherin beta-14) (Biotin); Protocadherin beta-14; anti-PCDHB14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PCDHB14 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCDHB14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the N-terminal region of human PCDHB14.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHB14 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHB14 antibody detected the PCDHB14 protein (arrow).)

Immunohistochemistry (IHC)

(PCDHB14 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB14 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PCDHB14 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCDHB14 antibody

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.

Product Categories/Family for anti-PCDHB14 antibody

Antibodies; Cadherin

References

Frank, M., et al. Curr. Opin. Cell Biol. 14(5):557-562(2002). Vanhalst, K., et al. FEBS Lett. 495 (1-2), 120-125 (2001): Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 9256608
• NCBI GeneID: 56122
• NCBI Accession #: NP_061757.1
• NCBI GenBank Nucleotide #: NM_018934.3
• UniProt Accession #: Q9Y5E9; Q4FZA4; Q4KN11
• Molecular Weight: 87,548 Da
• NCBI Official Full Name: protocadherin beta-14
• NCBI Official Synonym Full Names: protocadherin beta 14
• NCBI Official Symbol: PCDHB14
• NCBI Official Synonym Symbols: PCDH-BETA14
• NCBI Protein Information: protocadherin beta-14
• UniProt Protein Name: Protocadherin beta-14
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHB14
• UniProt Synonym Gene Names: PCDH-beta-14
• UniProt Entry Name: PCDBE_HUMAN

NCBI Description

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity.

Sequence similarities: Contains 6 cadherin domains.