Rabbit anti-Human PCDHA4 Polyclonal Antibody | anti-PCDHA4 antibody

PCDHA4, CT (PCDHA4, Protocadherin alpha-4) (FITC)

Cat. Num. AAA6331476

• Gene Names: PCDHA4; CNR1; CNRN1; CRNR1; PCDH-ALPHA4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHA4; Polyclonal Antibody; CT (PCDHA4; Protocadherin alpha-4) (FITC); Protocadherin alpha-4; anti-PCDHA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PCDHA4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PCDHA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 760-790 amino acids from the C-terminal region of human PCDHA4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHA4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PCDHA4 antibody detected the PCDHA4 protein (arrow).)

Related Product Information for anti-PCDHA4 antibody

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Product Categories/Family for anti-PCDHA4 antibody

Antibodies; Cadherin

References

Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000). Sugino, H., et al. Genomics 63(1):75-87(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 9256588
• NCBI GeneID: 56144
• NCBI Accession #: NP_061730.1; NP_113688.1
• NCBI GenBank Nucleotide #: NM_018907.3
• UniProt Accession #: Q9UN74; O75285; Q2M253
• Molecular Weight: 86,158 Da
• NCBI Official Full Name: protocadherin alpha-4 isoform 1
• NCBI Official Synonym Full Names: protocadherin alpha 4
• NCBI Official Symbol: PCDHA4
• NCBI Official Synonym Symbols: CNR1; CNRN1; CRNR1; PCDH-ALPHA4
• NCBI Protein Information: protocadherin alpha-4
• UniProt Protein Name: Protocadherin alpha-4
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHA4
• UniProt Synonym Gene Names: PCDH-alpha-4
• UniProt Entry Name: PCDA4_HUMAN

NCBI Description

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDHA4: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: integral to plasma membrane

Molecular Function: calcium ion binding

Biological Process: nervous system development; homophilic cell adhesion; cell adhesion