Rabbit PCAF Polyclonal Antibody | anti-Pcaf antibody

PCAF (p300/CBP-associated factor, CREBBP-associated factor, CAF, P/CAF)

Cat. Num. AAA610276

• Gene Names: Pcaf; Ada4; anon-WO0172774.89; CG4107; dGcn5; dGCN5; dGCN5 HAT; dKAT2; DmelCG4107; dmGCN5; dmHAG401; dPCAF; gcn5; Gcn5; GCN5; Gcn5/Pcaf; Gcn5/PCAF; p/CAF; P/CAF; PCAF
• Reactivity: Canine, Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PCAF; Polyclonal Antibody; PCAF (p300/CBP-associated factor; CREBBP-associated factor; CAF; P/CAF); Anti -PCAF (p300/CBP-associated factor; anti-Pcaf antibody

• Host: Rabbit
• Reactivity: Canine, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human and canine PCAF. Species Crossreactivity: Predicted to react with mouse and chicken due to sequence homology.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-Pcaf antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:500; Determined using whole extracts of human epidermoid carcinoma A431, canine MDCK kidney cells or recombinant active human PCAF, and a chemiluminescent detection reagent. Predicted molecular weight: ~88kD. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.; Immunoprecipitation: 5ug immunoprecipitates PCAF from RIPA lysate with ~4x10e7 human acute T cell leukemia Jurkat cells.
• Immunogen: Synthetic peptide [EKFFFSKIKEAGLIDK] (with cysteine added to N-terminal) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of human PCAF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Pcaf antibody

A direct link exists between hyperacetylation of chromatin and transcriptional activation. Experiments have established that PCAF possesses intrinsic histone acetylase activity. PCAF primarily acetylates lysine 14 of H3. It acetylates lysine 8 of H4 less efficiently. PCAF also acetylates p53 in response to DNA damage. The distinct patterns of acetylation by PCAF may contribute to transcriptional regulation of important genes.

Product Categories/Family for anti-Pcaf antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24663348
• NCBI GeneID: 39431
• NCBI Accession #: NP_648586.2
• NCBI GenBank Nucleotide #: NM_140329.3
• UniProt Accession #: Q9VTZ1
• Molecular Weight: 92,169 Da
• NCBI Official Full Name: pcaf
• NCBI Official Symbol: Pcaf
• NCBI Official Synonym Symbols: Ada4; anon-WO0172774.89; CG4107; dGcn5; dGCN5; dGCN5 HAT; dKAT2; DmelCG4107; dmGCN5; dmHAG401; dPCAF; gcn5; Gcn5; GCN5; Gcn5/Pcaf; Gcn5/PCAF; p/CAF; P/CAF; PCAF
• NCBI Protein Information: CG4107 gene product from transcript CG4107-RA; pcaf; CG4107-PA; Pcaf-PA
• UniProt Protein Name: GH11602p
• UniProt Gene Name: Pcaf
• UniProt Entry Name: Q9VTZ1_DROME