Western Blot (WB)
(PBXIP1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PBXIP1 antibody detected the PBXIP1 protein (arrow).)
Rabbit anti-Human PBXIP1 Polyclonal Antibody | anti-PBXIP1 antibody
PBXIP1, CT (PBXIP1, HPIP, Pre-B Cell leukemia transcription factor-interacting protein 1, Hematopoietic PBX-interacting protein) (APC)
Gene Names
PBXIP1; HPIP
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PBXIP1; Polyclonal Antibody; CT (PBXIP1; HPIP; Pre-B Cell leukemia transcription factor-interacting protein 1; Hematopoietic PBX-interacting protein) (APC); Pre-B-cell leukemia transcription factor-interacting protein 1; Hematopoietic PBX-interacting protein; anti-PBXIP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PBXIP1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PBXIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human PBXIP1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PBXIP1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PBXIP1 antibody detected the PBXIP1 protein (arrow).)
Western Blot (WB)
(PBXIP1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PBXIP1 antibody detected the PBXIP1 protein (arrow).)
Western Blot (WB)
(Western blot analysis of PBXIP1 (arrow) using rabbit polyclonal PBXIP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBXIP1 gene.)
Western Blot (WB)
(Western blot analysis of PBXIP1 (arrow) using rabbit polyclonal PBXIP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBXIP1 gene.)
Related Product Information for anti-PBXIP1 antibody
Regulator of pre-B-cell leukemia transcription factors (BPXs) function. Inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.
Product Categories/Family for anti-PBXIP1 antibody
References
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Lim, J., et al. Cell 125(4):801-814(2006). Abramovich, C., et al. Oncogene 21(44):6766-6771(2002). Abramovich, C., et al. J. Biol. Chem. 275(34):26172-26177(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,643 Da
NCBI Official Full Name
pre-B-cell leukemia transcription factor-interacting protein 1
NCBI Official Synonym Full Names
pre-B-cell leukemia homeobox interacting protein 1
NCBI Official Symbol
PBXIP1
NCBI Official Synonym Symbols
HPIP
NCBI Protein Information
pre-B-cell leukemia transcription factor-interacting protein 1; OTTHUMP00000035484; OTTHUMP00000035485; OTTHUMP00000035486; OTTHUMP00000035487; hematopoietic PBX-interacting protein; pre-B-cell leukemia transcription factor interacting protein 1
UniProt Protein Name
Pre-B-cell leukemia transcription factor-interacting protein 1
Protein Family
UniProt Gene Name
PBXIP1
UniProt Synonym Gene Names
HPIP
Uniprot Description
PBXIP1: Regulator of pre-B-cell leukemia transcription factors (BPXs) function. Inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytosol; microtubule; nucleus
Molecular Function: protein binding; transcription corepressor activity
Biological Process: cell differentiation; multicellular organism development; negative regulation of transcription, DNA-dependent
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Product Notes
The PBXIP1 pbxip1 (Catalog #AAA6331265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PBXIP1, CT (PBXIP1, HPIP, Pre-B Cell leukemia transcription factor-interacting protein 1, Hematopoietic PBX-interacting protein) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PBXIP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PBXIP1 pbxip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBXIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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