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Western Blot (WB) (Western blot analysis of UBXN11 expression in K562, mouse brain whole cell lysates.)

Rabbit PB Cadherin Polyclonal Antibody | anti-CDH antibody

PB Cadherin Antibody

Gene Names
CDH22; C20orf25; dJ998H6.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Immunogen affinity chromatography
Synonyms
PB Cadherin; Polyclonal Antibody; PB Cadherin Antibody; C20orf25; Cadherin-22; Pituitary and brain cadherin; PB-cadherin; anti-CDH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of PB Cadherin protein.
Purity/Purification
Immunogen affinity chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/mL (varies by lot)
Sequence Length
828
Applicable Applications for anti-CDH antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:300
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PB Cadherin.
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of UBXN11 expression in K562, mouse brain whole cell lysates.)

Western Blot (WB) (Western blot analysis of UBXN11 expression in K562, mouse brain whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of PB Cadherin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

Immunofluorescence (IF) (Immunofluorescent analysis of PB Cadherin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Product Categories/Family for anti-CDH antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
89 kDa
NCBI Official Full Name
cadherin-22
NCBI Official Synonym Full Names
cadherin 22
NCBI Official Symbol
CDH22
NCBI Official Synonym Symbols
C20orf25; dJ998H6.1
NCBI Protein Information
cadherin-22
UniProt Protein Name
Cadherin-22
UniProt Gene Name
CDH22
UniProt Synonym Gene Names
C20orf25; PB-cadherin

NCBI Description

This gene is a member of the cadherin superfamily. The gene product is composed of five cadherin repeat domains and a cytoplasmic tail similar to the highly conserved cytoplasmic region of classical cadherins. Expressed predominantly in the brain, this putative calcium-dependent cell adhesion protein may play an important role in morphogenesis and tissue formation in neural and non-neural cells during development and maintenance of the brain and neuroendocrine organs. [provided by RefSeq, Jul 2008]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. PB-cadherins may have a role in the morphological organization of pituitary gland and brain tissues ().

Research Articles on CDH

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Product Notes

The CDH cdh22 (Catalog #AAA9430919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PB Cadherin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PB Cadherin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:1000 IF: 1:100-1:300. Researchers should empirically determine the suitability of the CDH cdh22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PB Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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