Rabbit anti-Human PAX6 Polyclonal Antibody | anti-PAX6 antibody

PAX6 (PAX6, AN2, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin) (PE)

Cat. Num. AAA6331175

• Gene Names: PAX6; AN; AN1; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAX6; Polyclonal Antibody; PAX6 (PAX6; AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin) (PE); Oculorhombin; anti-PAX6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 436

• Applicable Applications for anti-PAX6 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PAX6 antibody is generated from rabbits immunized with a recombinant protein from human PAX6.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PAX6 (arrow) using rabbit polyclonal PAX6 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.)

Immunohistochemistry (IHC)

(PAX6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX6 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PAX6 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PAX6 antibody

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-PAX6 antibody

Antibodies; Transcription Factors

References

Gosmain, Y., et al. J. Biol. Chem. 285(43):33381-33393(2010). Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010). Zhang, X., et al. Cell Stem Cell 7(1):90-100(2010). Bremond-Gignac, D., et al. Mol. Vis. 16, 1705-1711 (2010): Cai, F., et al. Mol. Vis. 16, 1141-1145 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4580424
• NCBI GeneID: 5080
• NCBI Accession #: NP_001595.2; NP_001121084.1
• NCBI GenBank Nucleotide #: NP_001595.2
• UniProt Accession #: P26367
• NCBI Official Full Name: paired box protein Pax-6 isoform b
• NCBI Official Synonym Full Names: paired box 6
• NCBI Official Symbol: PAX6
• NCBI Official Synonym Symbols: AN; AN1; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
• NCBI Protein Information: paired box protein Pax-6
• UniProt Protein Name: Paired box protein Pax-6
• Protein Family: Paired box protein
• UniProt Gene Name: PAX6
• UniProt Synonym Gene Names: AN2
• UniProt Entry Name: PAX6_HUMAN

NCBI Description

This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain. [provided by RefSeq, Mar 2019]