Rabbit Pax-3a Polyclonal Antibody | anti-Pou4f1 antibody

Pax-3a, ID (Paired Box Gene 3, Transcription Factor PAX3, pax3a, Dr.75806, pax3, DKEY-20F20.1-001)

Cat. Num. AAA643747

• Gene Names: Pou4f1; Brn3; Brn-3; Brn3a; Brn3.0; Brn-3.0; E130119J07Rik
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Pax-3a; Polyclonal Antibody; ID (Paired Box Gene 3; Transcription Factor PAX3; pax3a; Dr.75806; pax3; DKEY-20F20.1-001); Anti -Pax-3a; anti-Pou4f1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes zebrafish Pax-3a.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-Pou4f1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: ELISA: 1:5000-1:20,000
• Immunogen: Synthetic peptide corresponding to the intermediate region of zebrafish Pax-3a.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Pou4f1 antibody

Paired box protein 3a (pax-3a) is a member of the paired box family of proteins. These genes are transcription factors with roles in developmental mechanisms. Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax-3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax-3/7-dependent cells generate satellite cells and most body muscle. Zebrafish pax3 is widely expressed in the lateral somite and, along with pax-7, becomes restricted anteriorly and then to the external cells on the lateral somite surface. Midline-derived Hedgehog signals appear to act directly on lateral somite cells to repress Pax-3/7. Both Hedgehog and Fgf-8, signals that induce muscle formation within the somite, suppress Pax-3/7 and promote expression of myogenic regulatory factors (MRFs) myf5 and myod in specific muscle precursor cell populations. Loss of MRF function leads to loss of myogenesis by specific populations of muscle fibres, with parallel up-regulation of Pax-3/7. The relationship between Pax-3/7 genes and myogenesis is evolutionarily ancient.

Product Categories/Family for anti-Pou4f1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 156938250
• NCBI GeneID: 18996
• NCBI Accession #: NP_035273.3
• NCBI GenBank Nucleotide #: NM_011143.4
• UniProt Accession #: P17208; E9QPT6
• Molecular Weight: 42,767 Da
• NCBI Official Full Name: POU domain, class 4, transcription factor 1
• NCBI Official Synonym Full Names: POU domain, class 4, transcription factor 1
• NCBI Official Symbol: Pou4f1
• NCBI Official Synonym Symbols: Brn3; Brn-3; Brn3a; Brn3.0; Brn-3.0; E130119J07Rik
• NCBI Protein Information: POU domain, class 4, transcription factor 1; brn-3A; brain-3A; brain-specific homeobox/POU domain protein 3A
• UniProt Protein Name: POU domain, class 4, transcription factor 1
• Protein Family: POU domain, class 4, transcription factor
• UniProt Gene Name: Pou4f1
• UniProt Synonym Gene Names: Brn-3; Brn3; Brn3a; Brain-3A; Brn-3A
• UniProt Entry Name: PO4F1_MOUSE

Uniprot Description

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Cellular Component: neuron projection; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; central nervous system neuron differentiation; trigeminal nerve development; transcription, DNA-dependent; positive regulation of apoptosis; multicellular organismal development; peripheral nervous system neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; habenula development; peripheral nervous system neuron development; cell migration in hindbrain; neuron differentiation; regulation of transcription from RNA polymerase II promoter; proprioception during equilibrioception; regulation of neurogenesis; suckling behavior; regulation of transcription, DNA-dependent; neuron fate specification; positive regulation of transcription from RNA polymerase II promoter; mesoderm development; sensory system development; negative regulation of neuron apoptosis