Rabbit anti-Human, Mouse PATJ Polyclonal Antibody | anti-PATJ antibody

PATJ Antibody

Cat. Num. AAA9631188

• Gene Names: INADL; Cipp; PATJ; hINADL; InaD-like
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PATJ; Polyclonal Antibody; PATJ Antibody; Channel interacting PDZ domain protein; Cipp; FLJ26982; hINADL; Inactivation no after potential D like protein; INAD like; InaD like Drosophila; INAD like protein; INADL; INADL protein; PALS 1 associated tight junction protein; PALS1 associated tight junction protein; PDZ domain protein; PDZ domain protein Drosophila inaD like; Post synaptic density 95 / discs large / zonula occludens 1 domain protein; Protein associated to tight junctions; anti-PATJ antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PATJ Antibody detects endogenous levels of PATJ.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PATJ antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PATJ.
• Conjugation: Unconjugated
• Similarity: The L27 domain (also called Maguk recruitment domain) is required for interaction with MPP5 and CRB3, and MPP5 localization to tight junctions. The PDZ domain 6 mediates interaction with the C-terminus of TJP3 and is crucial for localization to the tight junctions (PubMed:12021270). The PDZ domain 8 interacts with CLDN1 but is not required for proper localization (PubMed:12021270).
• Subunit Structure: Forms a ternary complex with MPP5, CRB1 and CRB3. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Forms a heterotrimeric complex composed of MMP5, LIN7B and PATJ; the N-terminal L27 domain of MPP5 interacts with the L27 domain of PATJ and the C-terminal L27 domain of MPP5 interacts with the L27 domain of LIN7B (By similarity). Interacts with TJP3/ZO-3 and CLDN1/claudin-1. Interacts with ASIC3, KCNJ10, KCNJ15, GRIN2A, GRIN2B, GRIN2C, GRIN2D, NLGN2, HTR2A and SLC6A4 (By similarity). Interacts with MPP7. Directly interacts with HTR4 (By similarity). Interacts (via PDZ domain 8) with WWC1 (via the ADDV motif). Interacts with SLC6A4 (By similarity). Interacts (via C-terminus) with ARHGEF18. Interacts with NPHP1.
• Tissue Specificity: Expressed in renal tubules (at protein level). Expressed in bladder, testis, ovary, small intestine, colon, heart, skeletal muscle, pancreas and cerebellum in the brain.
• Function: Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane (Probable). May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons (By similarity). May recruit ARHGEF18 to apical cell-cell boundaries.
• Note: Localizes to tight junctions in epithelial cells (PubMed:22006950). Also found at the apical plasma membrane (PubMed:11964389). Localized in the paranodal region of myelinating Schwann cells (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PATJ antibody

Cell junction; Tight junction; Apical cell membrane; Peripheral membrane protein; Cytoplasm; Perinuclear region; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 112382257
• NCBI GeneID: 10207
• NCBI Accession #: NP_795352.2
• NCBI GenBank Nucleotide #: NM_176877.2
• UniProt Accession #: Q8NI35; O15249; O43742; O60833; Q5VUA5; Q5VUA6; Q5VUA7; Q5VUA8; Q5VUA9; Q5VUB0; Q8WU78; Q9H3N9
• Molecular Weight: 125,231 Da
• NCBI Official Full Name: inaD-like protein
• NCBI Official Synonym Full Names: InaD-like (Drosophila)
• NCBI Official Symbol: INADL
• NCBI Official Synonym Symbols: Cipp; PATJ; hINADL; InaD-like
• NCBI Protein Information: inaD-like protein; PDZ domain protein; protein associated to tight junctions; channel-interacting PDZ domain protein; PALS1-associated tight junction protein; inactivation no after-potential D-like protein
• UniProt Protein Name: InaD-like protein
• Protein Family: Patj
• UniProt Gene Name: INADL
• UniProt Synonym Gene Names: PATJ; Inadl protein; hINADL
• UniProt Entry Name: INADL_HUMAN

NCBI Description

This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq, Jul 2008]

Uniprot Description

INADL: Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane. May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: tight junction; protein complex; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: intercellular junction assembly and maintenance