Immunostaining (IS)
(Paraffin-embedded kidney tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of P3113-82C4. Staining pattern appears to be restricted to proximal tubules with no distal tubule involvement.)
Rabbit anti-Human, Mouse Patched 1 Polyclonal Antibody | anti-PTCH1 antibody
Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1) (MaxLight 650)
WB: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate.
Applications are based on unconjugated antibody.
Immunostaining (IS)
(Paraffin-embedded kidney tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of P3113-82C4. Staining pattern appears to be restricted to proximal tubules with no distal tubule involvement.)
Immunostaining (IS)
(Paraffin-embedded kidney tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of P3113-82C4. Staining pattern appears to be restricted to proximal tubules with no distal tubule involvement.)
Testing Data
(Human skeletal muscle, fetal tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with P3113-82C4 (0.1ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates Protein Patched 1 (~160kD).)
Testing Data
(Human skeletal muscle, fetal tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with P3113-82C4 (0.1ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates Protein Patched 1 (~160kD).)
NCBI and Uniprot Product Information
Uniprot Description
PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.
Protein type: Cell cycle regulation; Tumor suppressor; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 9q22.3
Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; postsynaptic density; integral to membrane; plasma membrane; caveola; midbody
Molecular Function: heparin binding; cyclin binding; protein binding; hedgehog receptor activity; cholesterol binding; protein complex binding; patched binding; smoothened binding
Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to estradiol stimulus; response to chlorate; regulation of protein localization; negative regulation of osteoblast differentiation; negative regulation of epithelial cell proliferation; embryonic limb morphogenesis; response to drug; smoothened signaling pathway; response to retinoic acid; negative regulation of multicellular organism growth; pharyngeal system development; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; negative regulation of cell division; keratinocyte proliferation; spinal cord motor neuron differentiation; limb morphogenesis; organ morphogenesis; dorsal/ventral pattern formation; response to mechanical stimulus; ureteric bud branching; negative regulation of smoothened signaling pathway; neural plate pattern formation; neural tube closure; embryonic organ development; protein processing; brain development; regulation of smoothened signaling pathway
Disease: Holoprosencephaly 7; Basal Cell Nevus Syndrome; Basal Cell Carcinoma, Susceptibility To, 1
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Product Notes
The PTCH1 ptch1 (Catalog #AAA6492223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Patched 1 (PTCH, PTC, BCNS, FLJ42602, Holoprosencephaly 7, HPE7, NBCCS, Patched 1, Patched Homolog 1, Patched Protein Homolog 1, PTC1, PTCH1) (MaxLight 650) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Patched 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:300 detected Patched 1 in kidney tissue. Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended. WB: 0.1ug/ml detected Patched 1 on 10ug of human skeletal muscle, fetal tissue lysate. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PTCH1 ptch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Patched 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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