Western Blot (WB)
((1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PARP2 Polyclonal Antibody | anti-PARP2 antibody
Goat anti-PARP2 Antibody
Western Blot: Approx 70kDa band observed in Human Spleen lysates (calculated MW of 60.8kDa according to NP_005475.1. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
Uniprot Description
PARP2: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; EC 2.4.2.30; Transferase
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus
Molecular Function: DNA binding; DNA ligase (ATP) activity; NAD+ ADP-ribosyltransferase activity; protein binding
Biological Process: base-excision repair; DNA ligation during DNA repair; DNA repair; lagging strand elongation; protein amino acid ADP-ribosylation
Research Articles on PARP2
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Product Notes
The PARP2 parp2 (Catalog #AAA420891) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PARP2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 70kDa band observed in Human Spleen lysates (calculated MW of 60.8kDa according to NP_005475.1. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PARP2 parp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LDLFEVEKDG EKE. It is sometimes possible for the material contained within the vial of "PARP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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