Rabbit PARP1 / PARP Polyclonal Antibody | anti-PARP1 antibody

Rabbit Polyclonal to Human PARP1 / PARP

Cat. Num. AAA242586

• Gene Names: PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Monkey
• Applications: Immunohistochemistry, ELISA
• Purity: Protein G Purified
• Synonyms: PARP1 / PARP; Polyclonal Antibody; Rabbit Polyclonal to Human PARP1 / PARP; Anti-PARP1 / PARP Antibody (aa396-412) IHC-plus; PARP1; ADPRT; ADP-ribosyltransferase NAD(+); Adp-ribosyltransferase; ADPRT1; ARTD1; ADPRT 1; Poly(ADP-ribose) synthetase; Poly(ADP-ribosyl)transferase; PARP; Poly [ADP-ribose] polymerase 1; PADPRT-1; PARP-1; Poly (ADP-ribose) polymerase 1; Human PARP1; anti-PARP1 antibody

• Host: Rabbit
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Monkey
• Clonality: Polyclonal
• Specificity: KLH conjugated synthetic peptide comprising amino acids 396 - 412 [LTLGKLSRNKDEVKAMI] of the human ADP-ribosyltransferase (ADPRT) protein.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1014

• Applicable Applications for anti-PARP1 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:000 - 1:1000), IHC-P (5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PARP1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse (100%); Elephant (94%); Rat, Bovine, Rabbit, Opossum (88%); Mouse, Platypus (82%).
• Immunogen Type: Synthetic peptide
• Immunogen: PARP1 / PARP antibody was raised against synthetic peptide from human PARP1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse (100%); Elephant (94%); Rat, Bovine, Rabbit, Opossum (88%); Mouse, Platypus (82%).
• Antigen Modification: aa396-412
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-PARP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PARP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 156523968
• NCBI GeneID: 142
• NCBI Accession #: NP_001609.2
• NCBI GenBank Nucleotide #: NM_001618.3
• UniProt Accession #: P09874; Q8IUZ9; B1ANJ4
• Molecular Weight: 113,084 Da
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly (ADP-ribose) polymerase 1
• NCBI Official Symbol: PARP1
• NCBI Official Synonym Symbols: PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• NCBI Protein Information: poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: PARP1
• UniProt Synonym Gene Names: ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
• UniProt Entry Name: PARP1_HUMAN

NCBI Description

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Protein type: Nucleolus; Nuclear receptor co-regulator; EC 2.4.2.30; Nuclear envelope; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus

Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; transcription factor binding; protein kinase binding; NAD binding; NAD+ ADP-ribosyltransferase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; transforming growth factor beta receptor signaling pathway; double-strand break repair; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; telomere maintenance