Western Blot (WB)
(Western Blot Analysis of the Poly(ADP-ribose) polymerase, pAb . Lane 1: Molecular Weight Marker, Lane 2: Human HL60 Cell Extract .)
Rabbit PARP Polyclonal Antibody | anti-Poly(ADP-ribose) polymerase; PARP-1 antibody
PARP polyclonal antibody
Detects a band of ~116kDa by Western blot.
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.
Western Blot (WB)
(Western Blot Analysis of the Poly(ADP-ribose) polymerase, pAb . Lane 1: Molecular Weight Marker, Lane 2: Human HL60 Cell Extract .)
Western Blot (WB)
(Western Blot Analysis of the Poly(ADP-ribose) polymerase, pAb . Lane 1: Molecular Weight Marker, Lane 2: Human HL60 Cell Extract .)
NCBI and Uniprot Product Information
Uniprot Description
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner. Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.
Research Articles on Poly(ADP-ribose) polymerase; PARP-1
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Product Notes
The Poly(ADP-ribose) polymerase; PARP-1 parp1 (Catalog #AAA565256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP polyclonal antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's PARP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Western Blot (1:1,000, colorimetric). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application Detects a band of ~116kDa by Western blot. Researchers should empirically determine the suitability of the Poly(ADP-ribose) polymerase; PARP-1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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