Chromatin Immunoprecipitation (ChIP)
(PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.)
Rabbit anti-Human PARP-1 Polyclonal Antibody | anti-PARP-1 antibody
PARP-1 N-terminal antibody (pAb)
ChIP-Seq: 5ug per ChIP
IP: 10ug per IP
WB: 0.2-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Chromatin Immunoprecipitation (ChIP)
(PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.)
Chromatin Immunoprecipitation (ChIP)
(PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human NCI-H209 lung cancer cells and 5 ug of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.)
Western Blot (WB)
(PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 ug of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 ug/ml.)
Western Blot (WB)
(PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation. 10 ug of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ug of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 ug/ml.)
Western Blot (WB)
(PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.)
Western Blot (WB)
(PARP-1 N-terminal antibody (pAb) tested by Western blot. HeLa nuclear extract (25 ug) was probed with PARP-1 N-terminal antibody at a dilution of 1 ug/ml.)
Background: PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis. For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.
NCBI and Uniprot Product Information
Uniprot Description
PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
Protein type: Nucleolus; Nuclear envelope; DNA repair, damage; Nuclear receptor co-regulator; EC 2.4.2.30; Transferase
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; NAD binding; transcription factor binding; protein kinase binding; NAD+ ADP-ribosyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; transforming growth factor beta receptor signaling pathway; double-strand break repair; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of growth rate; telomere maintenance
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Product Notes
The PARP-1 parp1 (Catalog #AAA388990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP-1 N-terminal antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP-1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP-Seq: 5ug per ChIP IP: 10ug per IP WB: 0.2-1ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the PARP-1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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