Western Blot (WB)
(Anti-PARD6B rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Rabbit anti-Human PARD6B Polyclonal Antibody | anti-PARD6B antibody
Anti-PARD6B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
PARD6B; Polyclonal Antibody; Anti-PARD6B Antibody; Rabbit Polyclonal; PARD6B Antibody; Rabbit PAb; Antigen Affinity Purified; par-6 family cell polarity regulator beta; Anti-PAR6B Antibody; anti-PARD6B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PARD6B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PARD6B antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human PARD6B fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PARD6B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PARD6B rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-PARD6B rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PARD6B in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PARD6B in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-PARD6B antibody
PARD6B (Par-6 Family Cell Polarity Regulator Beta) is a Protein Coding gene. The PARD6B gene, located on 20q13.13, is conserved in chimpanzee, Rhesus monkey, cow, mouse, rat, chicken, zebrafish, and frog. This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain, and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cytoplasmic protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. PARD6B is widely expressed in the kidney, lung, and other tissues. Among its related pathways are G-protein signaling RAC1 in the cellular process and Ras signaling pathway. An important paralog of this gene is PARD6G.
References
Yosifova A, et al. (2011) Genome-wide association study on bipolar disorder in the bulgarian population. Genes Brain Behav 10 (7): 789-797.Michaux G, et al. (2016) The localisation of the apical par/cdc42 polarity module is specifically affected in microvillus inclusion disease. Biol Cell 108 (1): 19-28.Durgan J, et al. (2011) Par6b and atypical pkc regulate mitotic spindle orientation during epithelial morphogenesis. J Biol Chem 286 (14): 12461-12474.
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Product Notes
The PARD6B (Catalog #AAA8107997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PARD6B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARD6B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the PARD6B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARD6B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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