Rabbit PAR4 Polyclonal Antibody | anti-PAR4 antibody

PAR4 Polyclonal Antibody

Cat. Num. AAA3009849

• Gene Names: PAWR; PAR4; Par-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PAR4; Polyclonal Antibody; PAR4 Polyclonal Antibody; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Par-4; PAWR; anti-PAR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAR4 polyclonal antibody detects endogenous levels of PAR4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 340

• Applicable Applications for anti-PAR4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human PAR4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of PAR4 polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of PAR4 polyclonal antibody)

Related Product Information for anti-PAR4 antibody

Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper.

NCBI and Uniprot Product Information

• NCBI GI #: 55769533
• NCBI GeneID: 5074
• NCBI Accession #: NP_002574.2
• NCBI GenBank Nucleotide #: NP_002574.2
• UniProt Accession #: Q96IZ0
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: PRKC apoptosis WT1 regulator protein isoform 1
• NCBI Official Synonym Full Names: pro-apoptotic WT1 regulator
• NCBI Official Symbol: PAWR
• NCBI Official Synonym Symbols: PAR4; Par-4
• NCBI Protein Information: PRKC apoptosis WT1 regulator protein
• UniProt Protein Name: PRKC apoptosis WT1 regulator protein
• UniProt Gene Name: PAWR
• UniProt Synonym Gene Names: PAR4; Par-4
• UniProt Entry Name: PAWR_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases. [provided by RefSeq, Aug 2017]

Uniprot Description

PAR-4: a proapoptotic protein that drives trafficking and activation of Fas and Fasl to induce prostate cancer cell apoptosis and tumor regression. Capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappaB transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: cytoplasm; plasma membrane; actin filament; nucleus; actin cytoskeleton

Molecular Function: leucine zipper domain binding; protein binding; enzyme binding; actin binding; transcription corepressor activity

Biological Process: negative regulation of cell proliferation; actin filament bundle formation; negative regulation of T cell proliferation; interleukin-2 biosynthetic process; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of T cell receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of B cell proliferation; positive regulation of amyloid precursor protein biosynthetic process