Rabbit anti-Human PAPSS2 Polyclonal Antibody | anti-PAPSS2 antibody

PAPSS2, CT (PAPS Synthase 2, PAPSS 2, 3'-phosphoadenosine 5'-phosphosulfate Synthase 2, ATPSK2, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthase 2, Sulfurylase Kinase 2, SK 2, SK2) (APC)

Cat. Num. AAA6491967

• Gene Names: PAPSS2; SK2; BCYM4; ATPSK2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PAPSS2; Polyclonal Antibody; CT (PAPS Synthase 2; PAPSS 2; 3'-phosphoadenosine 5'-phosphosulfate Synthase 2; ATPSK2; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate Synthase 2; Sulfurylase Kinase 2; SK 2; SK2) (APC); ATP-sulfurylase; Sulfate Adenylate Transferase; Sulfate Adenylyltransferase; SAT; EC=2.7.7.4; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylyl-sulfate Kinase; Adenylylsulfate; 3'-phosphotransferase; APS Kinase; 3'-phosphoadenosine-5'-phosphosulfate Synthase; EC=2.7.1.25; anti-PAPSS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PAPSS2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PAPSS2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PAPSS2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PAPSS2 Pab is used in Western blot to detect PAPSS2 in Jurkat cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PAPSS2 antibody

Antibodies; Enzymes; Synthase

References

1. Xu, Z.H., et al., Biochem. Biophys. Res. Commun. 268(2):437-444 (2000). 2. Kurima, K., et al., J. Biol. Chem. 274(47):33306-33312 (1999). 3. ul Haque, M.F., et al., Nat. Genet. 20(2):157-162 (1998). 4. Kurima, K., et al., Proc. Natl. Acad. Sci. USA 95(15):8681-8685 (1998). 5. Shimizu, C., et al., Biochem. J. 363(Pt 2), 263-271 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 62912492
• NCBI GeneID: 9060
• NCBI Accession #: NP_001015880
• NCBI GenBank Nucleotide #: NM_001015880
• UniProt Accession #: O95340
• Molecular Weight: 70kDa
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 isoform b
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• NCBI Official Symbol: PAPSS2
• NCBI Official Synonym Symbols: SK2; BCYM4; ATPSK2
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS2
• UniProt Synonym Gene Names: ATPSK2; PAPS synthase 2; PAPSS 2; SK 2; SK2; SAT

NCBI Description

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth ().