Rabbit anti-Human, Mouse PAPSS1 Polyclonal Antibody | anti-PAPSS1 antibody

PAPSS1 Antibody

Cat. Num. AAA9629642

• Gene Names: PAPSS1; SK1; PAPSS; ATPSK1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PAPSS1; Polyclonal Antibody; PAPSS1 Antibody; anti-PAPSS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PAPSS1 Antibody detects endogenous levels of total PAPSS1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PAPSS1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The N-terminal first 50 residues are required for inhibition by the substrate adenylyl sulfate. In the N-terminal section; belongs to the APS kinase family. In the C-terminal section; belongs to the sulfate adenylyltransferase family.
• Subunit Structure: Homodimer.
• Tissue Specificity: Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.
• Function: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PAPSS1 antibody

Primary

NCBI and Uniprot Product Information

• NCBI GI #: 46094058
• NCBI GeneID: 9061
• NCBI Accession #: NP_005434.4
• NCBI GenBank Nucleotide #: NM_005443.4
• UniProt Accession #: O43252; O43841; O75332; Q96FB1; Q96TF4; Q9P1P9; Q9UE98
• Molecular Weight: 70,833 Da
• NCBI Official Full Name: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Synonym Full Names: 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• NCBI Official Symbol: PAPSS1
• NCBI Official Synonym Symbols: SK1; PAPSS; ATPSK1
• NCBI Protein Information: bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; SK 1; sulfurylase kinase 1
• UniProt Protein Name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
• Protein Family: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase
• UniProt Gene Name: PAPSS1
• UniProt Synonym Gene Names: ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT
• UniProt Entry Name: PAPS1_HUMAN

NCBI Description

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

Uniprot Description

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

Protein type: Kinase, other; Other Amino Acids Metabolism - selenoamino acid; Nucleotide Metabolism - purine; EC 2.7.1.25; Energy Metabolism - sulfur; EC 2.7.7.4

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytosol

Molecular Function: adenylylsulfate kinase activity; ATP binding; sulfate adenylyltransferase (ATP) activity; nucleotidyltransferase activity

Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; phosphorylation; skeletal development; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process