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Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.)

Rabbit anti-Human Palladin Polyclonal Antibody | anti-PALLD antibody

Palladin Antibody: ATTO 390

Gene Names
PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
Palladin; Polyclonal Antibody; Palladin Antibody: ATTO 390; Rabbit Anti-Human PALLD Polyclonal; Sarcoma antigen NY-SAR-77 Antibody; PALLD Antibody; CGI-151 Antibody; KIAA0992 Antibody; SIH002 Antibody; Palladin Antibody; anti-PALLD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Detects 150 kDa, 86 kDa. 86 kDa band is isoform 8.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
1123
Applicable Applications for anti-PALLD antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
PALLD
Immunogen
Synthetic peptide from the C-terminal of Human Palladin (aa. 1180-1280)
Immunogen Species
Human
Cellular Localization
Cytoplasm; Cytoskeleton; Cell Junction; Focal Adhesion; Cytoplasm; Myofibril; Sarcomere; Z Line; Cell Projection; Ruffle; Cell Projection; Podosome; Cell Projection; Lamellipodium; Cell Projection; Axon; Cell Projection; Growth Cone
Conjugate
ATTO 390
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines.
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.)

Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 150.5 kDa PALLD protein using Rabbit Anti-PALLD Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-PALLD Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 150.5 kDa. Other Band(s): 86 kDa.)
Product Categories/Family for anti-PALLD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,693 Da
NCBI Official Full Name
palladin isoform 1
NCBI Official Synonym Full Names
palladin, cytoskeletal associated protein
NCBI Official Symbol
PALLD
NCBI Official Synonym Symbols
MYN; PNCA1; CGI151; SIH002; CGI-151
NCBI Protein Information
palladin
UniProt Protein Name
Palladin
Protein Family
UniProt Gene Name
PALLD
UniProt Synonym Gene Names
KIAA0992

NCBI Description

This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.

Research Articles on PALLD

Similar Products

Product Notes

The PALLD palld (Catalog #AAA8001742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Palladin Antibody: ATTO 390 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Palladin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the PALLD palld for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Palladin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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